Overview

  • Product name

  • Description

    Rabbit polyclonal to BBOX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human BBOX1 aa 208-387. The exact sequence is proprietary.
    Sequence:

    LHH PPGVQLLHCI KQTVTGGDSE IVDGFNVCQK LKKNNPQAFQ ILSSTFVDFT DIGVDYCDFS VQSKHKIIEL DDKGQVVRIN FNNATRDTIF DVPVERVQPF YAALKEFVDL MNSKESKFTF KMNPGDVITF DNWRLLHGRR SYEAGTEISR HLEGAYADWD VVMSRLRILR QRVENGN


    Database link: O75936

  • Positive control

    • U251MG, MCF7, BT474, mouse liver, mouse kidney and mouse spinal cord cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab189827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    Catalyzes the formation of L-carnitine from gamma-butyrobetaine.
  • Tissue specificity

    Highly expressed in kidney; moderately expressed in liver; very low expression in brain.
  • Pathway

    Amine and polyamine biosynthesis; carnitine biosynthesis.
  • Sequence similarities

    Belongs to the gamma-BBH/TMLD family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2-oxoglutarate dioxygenase antibody
    • BBH antibody
    • BBOX 1 antibody
    • BBOX antibody
    • Bbox1 antibody
    • BODG_HUMAN antibody
    • Butyrobetaine (gamma) 2 oxoglutarate dioxygenase (gamma butyrobetaine hydroxylase) 1 antibody
    • G BBH antibody
    • Gamma BBH antibody
    • Gamma butyrobetaine 2 oxoglutarate dioxygenase 1 antibody
    • Gamma butyrobetaine dioxygenase antibody
    • Gamma butyrobetaine hydroxylase antibody
    • Gamma-BBH antibody
    • Gamma-butyrobetaine antibody
    • Gamma-butyrobetaine dioxygenase antibody
    • Gamma-butyrobetaine hydroxylase antibody
    see all

Images

  • All lanes : Anti-BBOX1 antibody (ab189827) at 1/500 dilution

    Lane 1 : U251MG cell extract
    Lane 2 : MCF7 cell extract
    Lane 3 : BT474 cell extract
    Lane 4 : Mouse liver cell extract
    Lane 5 : Mouse kidney cell extract
    Lane 6 : Mouse spinal cord cell extract

    Developed using the ECL technique.

    Predicted band size: 45 kDa

References

ab189827 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - Triton X 100
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 03 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up