Key features and details
- Rabbit polyclonal to BBS4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BBS4 antibody
See all BBS4 primary antibodies
DescriptionRabbit polyclonal to BBS4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human BBS4 aa 324-510. (BC027624)
FHFLSAAINFQPKMGELYMLLAVALTNLEDIENAKRAYAEAVHLDKCNPL VNLNYAVLLYNQGEKKNALAQYQEMEKKVSLLKDNSSLEFDSEMVEMAQK LGAALQVGEALVWTKPVKDPKSKHQTTSTSKPASFQQPLGSNQALGQAMS SAAAYRTLPSGAGGTSQFTKPPSLPLEPEP AVESSPT
Database link: Q96RK4
- HeLa and 293 cell lysates; Human fetal pancreas tissue.
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FormLyophilized:Reconstitute in 200ul sterile H2O.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/200 - 1/1000. Predicted molecular weight: 58 kDa.|
FunctionMay be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.
Tissue specificityUbiquitously expressed. The highest level of expression is found in the kidney.
Involvement in diseaseDefects in BBS4 are the cause of Bardet-Biedl syndrome type 4 (BBS4) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect.
Sequence similaritiesBelongs to the BBS4 family.
Contains 10 TPR repeats.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton. Cell projection > cilium membrane. Cytoplasm. Localizes to the pericentriolar region throughout the cell cycle. Centrosomal localization requires dynein. Localizes to nonmembranous centriolar satellites in the cytoplasm.
- Information by UniProt
- Bardet Biedl syndrome 4 protein antibody
- Bardet-Biedl syndrome 4 protein antibody
- Bbs4 antibody
- BBS4_HUMAN antibody
ab197122 has not yet been referenced specifically in any publications.