Overview

  • Product name

  • Description

    Rabbit polyclonal to BBS9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 190-239 (TDSFLTVSSC QQVESYKYQV LAFATDADKR QETEQQKLGS GKRLVVDWTL) of Human BBS9 (NP_055266).

  • Positive control

    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab133841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa.

Target

  • Function

    The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.
  • Tissue specificity

    Widely expressed. Expressed in adult heart, skeletal muscle, lung, liver, kidney, placenta and brain, and in fetal kidney, lung, liver and brain.
  • Involvement in disease

    A chromosomal aberration involving PTHB1 is found in Wilms tumor 5 (WT5) [MIM:601583]. Translocation t(1;7)(q42;p15) with OBSCN.
    Defects in BBS9 are a cause of Bardet-Biedl syndrome type 9 (BBS9) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium membrane. Cytoplasm. Localizes to nonmembranous centriolar satellites in the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • B1 antibody
    • Bardet Biedl syndrome 9 antibody
    • Bardet-Biedl syndrome 9 protein antibody
    • bbs9 antibody
    • C18 antibody
    • D1 antibody
    • MGC118917 antibody
    • OTTHUMP00000158833 antibody
    • OTTHUMP00000202918 antibody
    • OTTHUMP00000202919 antibody
    • OTTHUMP00000202920 antibody
    • Parathyroid hormone-responsive B1 gene protein antibody
    • Protein PTHB1 antibody
    • PTH-responsive osteosarcoma B1 protein antibody
    • PTHB1 antibody
    • PTHB1_HUMAN antibody
    see all

Images

  • Anti-BBS9 antibody (ab133841) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size: 95 kDa

References

ab133841 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - other (oocytes)
Gel Running Conditions
Reduced Denaturing (4-15% gel)
Loading amount
1.08 µg
Specification
oocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Addie Johnston

Verified customer

Submitted Mar 28 2018

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