BCA disodium salt, protein concentration determination reagent (ab146331)

Overview

  • Product name

    BCA disodium salt, protein concentration determination reagent
  • Description

    Protein concentration determination reagent.
  • Alternative names

    • 2,2'-Biquinoline-4,4'-dicarboxylic acid disodium salt
    • Bicinchonic acid, disodium salt
  • Biological description

    Cu(I)-specific colorimetric reagent used for measuring protein concentration. Proteins reduce alkaline Cu(II) to Cu(I) which complexes with BCA to form a purple complex. Absorbance at 562nm is directly proportional to protein concentration.

  • Purity

    > 98%
  • CAS Number

    979-88-4
  • Chemical structure

    Chemical Structure

Properties

  • Molecular weight

    388.30
  • Molecular formula

    C20H10N2Na2O4
  • Storage instructions

    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Johnson DK  et al. Stabilization of Cu(I) for binding and calorimetric measurements in aqueous solution. Dalton Trans 44:16494-505 (2015). Read more (PubMed: 26327397) »
  • Wang YX  et al. Differentiation of bel-7402 human hepatocarcinoma cells induced by aqueous extracts of fresh gecko (AG) and its anti-tumor activity in vivo. J Ethnopharmacol 155:1583-8 (2014). Read more (PubMed: 25093547) »

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