Product nameBCA disodium salt, protein concentration determination reagent
DescriptionProtein concentration determination reagent.
- 2,2'-Biquinoline-4,4'-dicarboxylic acid disodium salt
- Bicinchonic acid, disodium salt
Cu(I)-specific colorimetric reagent used for measuring protein concentration. Proteins reduce alkaline Cu(II) to Cu(I) which complexes with BCA to form a purple complex. Absorbance at 562nm is directly proportional to protein concentration.
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Johnson DK et al. Stabilization of Cu(I) for binding and calorimetric measurements in aqueous solution. Dalton Trans 44:16494-505 (2015). Read more (PubMed: 26327397) »
- Wang YX et al. Differentiation of bel-7402 human hepatocarcinoma cells induced by aqueous extracts of fresh gecko (AG) and its anti-tumor activity in vivo. J Ethnopharmacol 155:1583-8 (2014). Read more (PubMed: 25093547) »