Product nameBCA Protein Assay Kit
See all BCA Protein kits
Sample typeSerum, Plasma, Cell culture extracts, Tissue Extracts
Sensitivity> 0.5 µg
Range0.5 µg - 30 µg
Assay time1h 30m
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu2+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 µg protein (0.01-0.6 mg/ml).
This kit was previously called BCA Protein Quantification Kit.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1000 tests BCA Reagent NM 1 x 100ml BSA Standard Yellow 1 x 1ml Copper Reagent Blue 1 x 2ml
RelevanceThe BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration.
- Bicinchoninic acid
- Bicinchoninic acid protein
This product has been referenced in:
- Wang K et al. Signal transducer and activator of transcription 3 inhibition enhances vemurafenib sensitivity in colon cancers harboring the BRAFV600E mutation. J Cell Biochem 120:5315-5325 (2019). Read more (PubMed: 30320916) »
- Li S et al. Quercetin suppresses the proliferation and metastasis of metastatic osteosarcoma cells by inhibiting parathyroid hormone receptor 1. Biomed Pharmacother 114:108839 (2019). Read more (PubMed: 30978523) »