BCA protein assay kit for low concentrations (ab207002)

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Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).
  • Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell Lysate, Purified protein, Tissue Lysate

Overview

  • Product name

    BCA protein assay kit for low concentrations
    See all BCA Protein kits

  • Detection method

    Colorimetric

  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate

  • Product overview

    Abcam’s BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.


    The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The Cu1+-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 µg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.


    Support on how to determine protein concentration can be found here.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 2500 tests
    BCA Reagent A 1 x 190ml
    BCA Reagent B 1 x 190ml
    BCA Reagent C 1 x 9ml
    BSA 5 x 1ml

  • Research areas

  • Relevance

    The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration.

  • Alternative names

    • BCA
    • Bicinchoninic acid
    • Bicinchoninic acid protein

Images

  • Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).
    Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).

    Typical standard curve obtained for BSA and Cytochrome C from bovine heart using ab207002 and the microplate procedure (37°C for 2 hours).

     

  • Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).
    Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).

    Typical standard curve obtained for BSA and Cytochrome C from bovine heart by using ab207002 and the test tube procedure (60°C for 1 hour).

References (8)

Publishing research using ab207002? Please let us know so that we can cite the reference in this datasheet.

ab207002 has been referenced in 8 publications.

  • Takov K  et al. Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential. Basic Res Cardiol 115:26 (2020). PubMed: 32146560
  • Takov K  et al. Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential. J Extracell Vesicles 8:1560809 (2019). PubMed: 30651940
  • Qin QJ  et al. Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis. Mol Med Rep 19:2581-2590 (2019). PubMed: 30720139
  • Austin Suthanthiraraj PP & Sen AK Localized surface plasmon resonance (LSPR) biosensor based on thermally annealed silver nanostructures with on-chip blood-plasma separation for the detection of dengue non-structural protein NS1 antigen. Biosens Bioelectron 132:38-46 (2019). PubMed: 30851494
  • Yuan X  et al. Aldosterone promotes renal interstitial fibrosis via the AIF-1/AKT/mTOR signaling pathway. Mol Med Rep 20:4033-4044 (2019). PubMed: 31545432
  • Salah MM  et al. Pirfenidone alleviates concanavalin A-induced liver fibrosis in mice. Life Sci 239:116982 (2019). PubMed: 31639402
  • Li Y  et al. Upregulation of allograft inflammatory factor-1 expression and secretion by macrophages stimulated with aldosterone promotes renal fibroblasts to a profibrotic phenotype. Int J Mol Med 42:861-872 (2018). PubMed: 29749461
  • Takov K  et al. Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes. J Extracell Vesicles 6:1388731 (2017). PubMed: 29184625

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