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    bca-protein-assay-kit-for-low-concentrations-ab207002.pdf

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BCA protein assay kit for low concentrations (ab207002)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).
  • Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell Lysate, Purified protein, Tissue Lysate

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Overview

  • Product name

    BCA protein assay kit for low concentrations
    See all BCA Protein kits
  • Detection method

    Colorimetric
  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate
  • Product overview

    Abcam’s BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.


    The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The Cu1+-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 µg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.


    Support on how to determine protein concentration can be found here.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K814 Extra Sense™ BCA Protein Assay Kit. K814-2500 is the same size as the 2500 test size of ab207002.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 2500 tests
    BCA Reagent A 1 x 190ml
    BCA Reagent B 1 x 190ml
    BCA Reagent C 1 x 9ml
    BSA 5 x 1ml
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Protein quantification kits
  • Relevance

    The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration.
  • Alternative names

    • BCA
    • Bicinchoninic acid
    • Bicinchoninic acid protein

Associated products

  • Related Products

    • Bradford Assay Kit (ab102535)
    • BCA Protein Assay Kit (ab102536)

Images

  • Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).
    Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).

    Typical standard curve obtained for BSA and Cytochrome C from bovine heart using ab207002 and the microplate procedure (37°C for 2 hours).

     

  • Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).
    Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).

    Typical standard curve obtained for BSA and Cytochrome C from bovine heart by using ab207002 and the test tube procedure (60°C for 1 hour).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (11)

Publishing research using ab207002? Please let us know so that we can cite the reference in this datasheet.

ab207002 has been referenced in 11 publications.

  • Gericke A  et al. Sulodexide reduces glucose induced senescence in human retinal endothelial cells. Sci Rep 11:11532 (2021). PubMed: 34075143
  • Takov K  et al. Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential. Basic Res Cardiol 115:26 (2020). PubMed: 32146560
  • Wójtowicz J  et al. Compensation Mechanism of the Photosynthetic Apparatus in Arabidopsis thaliana ch1 Mutants. Int J Mol Sci 22:N/A (2020). PubMed: 33379339
  • Busche M  et al. HepaChip-MP - a twenty-four chamber microplate for a continuously perfused liver coculture model. Lab Chip 20:2911-2926 (2020). PubMed: 32662810
  • Takov K  et al. Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential. J Extracell Vesicles 8:1560809 (2019). PubMed: 30651940
View all Publications for this product

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