Overview

  • Product name

    BCA protein assay kit for low concentrations
    See all BCA Protein kits
  • Detection method

    Colorimetric
  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate
  • Product overview

    Abcam’s BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.


    The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The Cu1+-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 µg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 2500 tests
    BCA Reagent A 1 x 190ml
    BCA Reagent B 1 x 190ml
    BCA Reagent C 1 x 9ml
    BSA 5 x 1ml
  • Research areas

  • Relevance

    The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration.
  • Alternative names

    • BCA
    • Bicinchoninic acid
    • Bicinchoninic acid protein

Images

Protocols

References

This product has been referenced in:

  • Takov K  et al. Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential. J Extracell Vesicles 8:1560809 (2019). Read more (PubMed: 30651940) »
  • Qin QJ  et al. Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis. Mol Med Rep 19:2581-2590 (2019). Read more (PubMed: 30720139) »
See all 4 Publications for this product

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