Overview

  • Product name

    BCA protein assay kit reducing agent compatible (microplate)
    See all BCA Protein kits
  • Detection method

    Colorimetric
  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate
  • Product overview

    Abcam’s BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.


    Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM and β-mercaptoethanol up to 35 mM. This kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The assay is linear over a wide range of protein concentrations (25-2000 μg/mL). The kit also includes Bovine Serum Albumin (BSA) as a reference protein standard. The kit has been formatted for microplate-based protein assays.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 1000 tests
    BCA Reagent A 1 x 200ml
    BCA Reagent B 1 x 20ml
    Blocking Reagent 20 vials
    Blocking Reagent Buffer 1 x 20ml
    BSA 10 x 1ml
  • Research areas

  • Relevance

    The BCA Protein assay (or Smith assay) is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is determined by measuring the color change of the sample solution from green to purple which is proportional to the protein concentration.
  • Alternative names

    • BCA
    • Bicinchoninic acid
    • Bicinchoninic acid protein

Images

Protocols

References

This product has been referenced in:

  • Zhang G  et al. Neuroprotective Effect of Astragalus Polysacharin on Streptozotocin (STZ)-Induced Diabetic Rats. Med Sci Monit 25:135-141 (2019). Read more (PubMed: 30610831) »
  • Huang J  et al. HMGB1 Mediates Autophagy Dysfunction via Perturbing Beclin1-Vps34 Complex in Dopaminergic Cell Model. Front Mol Neurosci 10:13 (2017). Read more (PubMed: 28197072) »
See all 2 Publications for this product

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