Overview

  • Product name

    Anti-BCA225 antibody [CU18]
  • Description

    Mouse monoclonal [CU18] to BCA225
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    BCA 225 protein secreted by the T47D (Clone 11) human breast carcinoma cellline.

  • Positive control

    • Breast carcinoma (human).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    CU18
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notes
    Immunohistochemistry: 1/40.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    Antigen retrieval is not essential, although may be beneficial.

    Strong intracytoplasmic staining is seen in primary and metastatic breast carcinomatissue, as well as in cervical carcinomas. Apical/glycocalyx staining isseen in normal kidney, lung, fallopian tube, liver, skin (eccrine sweat glands)and uterus.
    Similar staining patterns are observed in lung, ovarian, and endometrialcancers.
    Carcinomas of the colon, stomach, prostate, urinary bladder,liver, pancreas, thyroid, and parotid are negative, as are sarcomas and lymphoidcancers.
  • Target

    • Relevance

      BCA225 is a 225 kDa glycoprotein secreted by the T47D breast carcinoma cell line.
    • Alternative names

      • BRST1 antibody

    References

    This product has been referenced in:

    • Nishikata T  et al. Primary culture of breast cancer: a model system for epithelial-mesenchymal transition and cancer stem cells. Anticancer Res 33:2867-73 (2013). Read more (PubMed: 23780972) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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