Key features and details
- Mouse monoclonal [CU18] to BCA225
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-BCA225 antibody [CU18]
DescriptionMouse monoclonal [CU18] to BCA225
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
BCA 225 protein secreted by the T47D (Clone 11) human breast carcinoma cellline.
- Breast carcinoma (human).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab3360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
Antigen retrieval is not essential, although may be beneficial.
Strong intracytoplasmic staining is seen in primary and metastatic breast carcinomatissue, as well as in cervical carcinomas. Apical/glycocalyx staining isseen in normal kidney, lung, fallopian tube, liver, skin (eccrine sweat glands)and uterus.
Similar staining patterns are observed in lung, ovarian, and endometrialcancers.
Carcinomas of the colon, stomach, prostate, urinary bladder,liver, pancreas, thyroid, and parotid are negative, as are sarcomas and lymphoidcancers.
RelevanceBCA225 is a 225 kDa glycoprotein secreted by the T47D breast carcinoma cell line.
- BRST1 antibody
ab3360 has been referenced in 1 publication.
- Nishikata T et al. Primary culture of breast cancer: a model system for epithelial-mesenchymal transition and cancer stem cells. Anticancer Res 33:2867-73 (2013). PubMed: 23780972