Anti-BCAR1 antibody (ab137507)
Key features and details
- Rabbit polyclonal to BCAR1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BCAR1 antibody
See all BCAR1 primary antibodies -
Description
Rabbit polyclonal to BCAR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 250-710 of Human BCAR1 (UniProt ID: P56945).
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Positive control
- A431, H1299, HeLa, HepG2 and NIH 3T3 cell lysates; methanol fixed A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 93 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. -
Tissue specificity
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line. -
Sequence similarities
Belongs to the CAS family.
Contains 1 SH3 domain. -
Domain
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
Post-translational
modificationsPTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
Cellular localization
Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 527550 Cow
- Entrez Gene: 9564 Human
- Entrez Gene: 12927 Mouse
- Entrez Gene: 25414 Rat
- Omim: 602941 Human
- SwissProt: P56945 Human
- SwissProt: Q61140 Mouse
- SwissProt: Q63767 Rat
see all -
Alternative names
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
Images
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Anti-BCAR1 antibody (ab137507) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Anti-BCAR1 antibody (ab137507) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Confocal immunofluorescence analysis of methanol fixed A431 cells labelling BCAR1 with ab137507 (green) at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137507 has not yet been referenced specifically in any publications.