Overview

  • Product name

  • Description

    Rabbit polyclonal to BCAR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 250-710 of Human BCAR1 (UniProt ID: P56945).

  • Positive control

    • A431, H1299, HeLa, HepG2 and NIH 3T3 cell lysates; methanol fixed A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • Tissue specificity

    Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • Sequence similarities

    Belongs to the CAS family.
    Contains 1 SH3 domain.
  • Domain

    Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • Post-translational
    modifications

    PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • Cellular localization

    Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all

Images

  • Anti-BCAR1 antibody (ab137507) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 93 kDa



    7.5% SDS PAGE
  • Anti-BCAR1 antibody (ab137507) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 93 kDa



    7.5% SDS PAGE
  • Confocal immunofluorescence analysis of methanol fixed A431 cells labelling BCAR1 with ab137507 (green) at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/500 dilution.

References

ab137507 has not yet been referenced specifically in any publications.

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