Recombinant Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free (ab247628)
![Western blot - Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free (ab247628)](https://www.abcam.com/ps/products/247/ab247628/Images/ab247628-398058-anti-bcar1-antibody-epr1850-2-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free (ab247628)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1850(2)] to BCAR1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free
See all BCAR1 primary antibodies -
Description
Rabbit monoclonal [EPR1850(2)] to BCAR1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human BCAR1. The exact sequence is proprietary.
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General notes
Ab247628 is the carrier-free version of ab108320. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247628 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR1850(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 93 kDa. |
Target
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Function
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. -
Tissue specificity
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line. -
Sequence similarities
Belongs to the CAS family.
Contains 1 SH3 domain. -
Domain
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
Post-translational
modificationsPTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
Cellular localization
Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 9564 Human
- Entrez Gene: 12927 Mouse
- Entrez Gene: 25414 Rat
- Omim: 602941 Human
- SwissProt: P56945 Human
- SwissProt: Q61140 Mouse
- SwissProt: Q63767 Rat
- Unigene: 479747 Human
see all -
Alternative names
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
Images
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Western blot - Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free (ab247628)All lanes : Anti-BCAR1 antibody [EPR1850(2)] (ab108320) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : A431 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDaThis data was developed using ab108320, the same antibody clone in a different buffer formulation.
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![Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free (ab247628)](https://www.abcam.com/ps/products/247/ab247628/Images/ab247628-2-benefits-of-recombinant-antibodies.png)
Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free (ab247628)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247628 has not yet been referenced specifically in any publications.
