Recombinant
RabMAb

Recombinant Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free (ab247628)

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Overview

  • Product name

    Anti-BCAR1 antibody [EPR1850(2)] - BSA and Azide free
    See all BCAR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1850(2)] to BCAR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human BCAR1. The exact sequence is proprietary.

  • General notes

    Ab247628 is the carrier-free version of ab108320. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247628 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab247628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

    • Tissue specificity

      Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.

    • Sequence similarities

      Belongs to the CAS family.
      Contains 1 SH3 domain.

    • Domain

      Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
      A serine-rich region promotes activation of the serum response element (SRE).
      The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.

    • Post-translational
      modifications

      PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
      Dephosphorylated by PTPN14 at Tyr-128.

    • Cellular localization

      Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BCAR 1 antibody
      • Bcar1 antibody
      • BCAR1_HUMAN antibody
      • Breast cancer anti estrogen resistance 1 antibody
      • Breast cancer anti estrogen resistance 1 protein antibody
      • Breast cancer anti-estrogen resistance protein 1 antibody
      • CAS antibody
      • Cas scaffolding protein family member 1 antibody
      • CAS1 antibody
      • Cass1 antibody
      • Crk associated substrate antibody
      • Crk associated substrate p130Cas antibody
      • CRK-associated substrate antibody
      • CRKAS antibody
      • FLJ12176 antibody
      • FLJ45059 antibody
      • p130cas antibody
      see all

    References

    ab247628 has not yet been referenced specifically in any publications.

    Publishing research using ab247628? Please let us know so that we can cite the reference in this datasheet.

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