Product nameAnti-BCAR1 (phospho Y751) antibody
See all BCAR1 primary antibodies
DescriptionRabbit polyclonal to BCAR1 (phospho Y751)
SpecificityThe antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. .
- Pervanadate treated human endothelial and A431 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier).
Our Abpromise guarantee covers the use of ab45486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 93 kDa.|
FunctionDocking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Tissue specificityWidely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
Sequence similaritiesBelongs to the CAS family.
Contains 1 SH3 domain.
DomainContains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
modificationsPTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128.
Cellular localizationCell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
- Information by UniProt
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
Lanes 1-2 : anti-p130 Cas
Lanes 3-4 : Anti-BCAR1 (phospho Y751) antibody (ab45486) at 1/2000 dilution
Lanes 5-6 : anti-p130 Cas (Tyr-762)
Lanes 1 & 3 & 5 : human endothelial cells serum starved overnight
Lanes 2 & 4 & 6 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
Predicted band size: 93 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ab45486 has not yet been referenced specifically in any publications.