• Product name
    Anti-BCAR1 (phospho Y762) antibody
    See all BCAR1 primary antibodies
  • Description
    Rabbit polyclonal to BCAR1 (phospho Y762)
  • Host species
  • Specificity
    This antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated BCAR1 on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src binding domain of rat BCAR1. This rat sequence is the same as Tyr-664 in human BCAR1 and Tyr-664 in mouse BCAR1. This site is also highly conserved in Cas-L (HEF1, Nedd9).


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-BCAR1(Tyr-751) peptide before affinity purification using phospho-BCAR1(Tyr-762) peptide (without carrier).
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab41827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 130 kDa.
ELISA 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 22084245


  • Function
    Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • Tissue specificity
    Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • Sequence similarities
    Belongs to the CAS family.
    Contains 1 SH3 domain.
  • Domain
    Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • Post-translational
    PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • Cellular localization
    Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all


  • Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4-6). The blot was probed with anti-BCAR1 (lanes 1 & 2) or anti-BCAR1 (Tyr-762) (lanes 3-6). The latter was used in the presence of no peptide (lane 4), phospho-p130 Cas (Tyr-762) peptide (lane 5) or phospho-BCAR1 (Tyr-751) peptide (lane 6).
  • ab41827 staining BCAR1 in stable PC3 cells by Immunocytochemistry/ Immunofluorescence. Cells were counterstained with DAPI (blue).


This product has been referenced in:
  • Zheng Y  et al. Protein-tyrosine Kinase 6 Promotes Peripheral Adhesion Complex Formation and Cell Migration by Phosphorylating p130 CRK-associated Substrate. J Biol Chem 287:148-58 (2012). ICC/IF ; Human . Read more (PubMed: 22084245) »
See 1 Publication for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Human Cell (Prostate cancer cell PC3)
Prostate cancer cell PC3
Carnoy’s solution
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

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Submitted Feb 28 2012

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