Anti-BCAR3/NSP2 antibody (ab85940)
Key features and details
- Rabbit polyclonal to BCAR3/NSP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-BCAR3/NSP2 antibody -
Description
Rabbit polyclonal to BCAR3/NSP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human BCAR3/NSP2 aa 1-100. NP_003558.1
Database link: O75815 -
Positive control
- HeLa and 293T whole cell lysates
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab85940 was affinity purified using an epitope specific to BCAR3/NSP2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
-
Function
May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. -
Tissue specificity
Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue. -
Sequence similarities
Contains 1 Ras-GEF domain.
Contains 1 SH2 domain. - Information by UniProt
-
Database links
- Entrez Gene: 8412 Human
- Omim: 604704 Human
- SwissProt: O75815 Human
- Unigene: 36958 Human
-
Alternative names
- BCAR 3 antibody
- BCAR3 antibody
- BCAR3_HUMAN antibody
see all
Images
-
All lanes : Anti-BCAR3/NSP2 antibody (ab85940) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 300 kDa, 460 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of human BCAR3/NSP2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85940 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab85940 has not yet been referenced specifically in any publications.