Key features and details
- Rabbit polyclonal to BCAR3/NSP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BCAR3/NSP2 antibody
See all BCAR3/NSP2 primary antibodies
DescriptionRabbit polyclonal to BCAR3/NSP2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human BCAR3/NSP2 aa 25-75.
Database link: NP_003558.1
- HeLa and 293T whole cell lysates
This product was previously labelled as BCAR3
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85940 was affinity purified using an epitope specific to BCAR3/NSP2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
Tissue specificityUbiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue.
Sequence similaritiesContains 1 Ras-GEF domain.
Contains 1 SH2 domain.
- Information by UniProt
- BCAR 3 antibody
- BCAR3 antibody
- BCAR3_HUMAN antibody
All lanes : Anti-BCAR3/NSP2 antibody (ab85940) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 300 kDa, 460 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of human BCAR3/NSP2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85940 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab85940 has not yet been referenced specifically in any publications.