Anti-BCAS2 antibody (ab151293)

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Western blot - Anti-BCAS2 antibody (ab151293)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS2 antibody (ab151293)
  • Immunocytochemistry/ Immunofluorescence - Anti-BCAS2 antibody (ab151293)

Key features and details

  • Rabbit polyclonal to BCAS2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-BCAS2 antibody
    See all BCAS2 primary antibodies

  • Description

    Rabbit polyclonal to BCAS2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 19-204 of Human BCAS2

  • Positive control

    • NT2D1 cells; IMR32 cells; MCF-7 cell lysate; HeLa cells; J5 xenograft.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab151293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in mRNA splicing.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the SPF27 family.

  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • bcas2 antibody
    • Breast carcinoma amplified sequence 2 antibody
    • Breast carcinoma-amplified sequence 2 antibody
    • DAM 1 antibody
    • DAM-1 antibody
    • DAM1 antibody
    • DNA amplified in mammary carcinoma 1 protein antibody
    • MGC7712 antibody
    • Pre mRNA splicing factor SPF27 antibody
    • Pre-mRNA-splicing factor spf27 antibody
    • Snt309 antibody
    • SPF27 antibody
    • SPF27_HUMAN antibody
    • Spliceosome associated protein amplified in breast cancer antibody
    • Spliceosome associated protein SPF 27 antibody
    • Spliceosome-associated protein SPF 27 antibody
    see all

Images

  • Western blot - Anti-BCAS2 antibody (ab151293)
    Western blot - Anti-BCAS2 antibody (ab151293)
    Anti-BCAS2 antibody (ab151293) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS2 antibody (ab151293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS2 antibody (ab151293)
    Immunohistochemical analysis of paraffin-embedded J5 xenograft labeling BCAS2 with ab151293 antibody at 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-BCAS2 antibody (ab151293)
    Immunocytochemistry/ Immunofluorescence - Anti-BCAS2 antibody (ab151293)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling BCAS2 with ab151293 antibody at 1/200 dilution. Bottom figure represents cells costained with Hoechst 33342.

References (1)

Publishing research using ab151293? Please let us know so that we can cite the reference in this datasheet.

ab151293 has been referenced in 1 publication.

  • Lang B & Zhao S miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2. Oncol Rep 39:71-80 (2018). PubMed: 29115564

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