Anti-BCAS2 antibody (ab151293)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-BCAS2 antibody
See all BCAS2 primary antibodies -
Description
Rabbit polyclonal to BCAS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 19-204 of Human BCAS2
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Positive control
- NT2D1 cells; IMR32 cells; MCF-7 cell lysate; HeLa cells; J5 xenograft.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab151293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Involved in mRNA splicing. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the SPF27 family. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 10286 Human
- Entrez Gene: 68183 Mouse
- Entrez Gene: 295334 Rat
- Omim: 605783 Human
- SwissProt: O75934 Human
- SwissProt: Q9D287 Mouse
- Unigene: 22960 Human
- Unigene: 104919 Mouse
see all -
Alternative names
- bcas2 antibody
- Breast carcinoma amplified sequence 2 antibody
- Breast carcinoma-amplified sequence 2 antibody
see all
Images
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Western blot - Anti-BCAS2 antibody (ab151293)Anti-BCAS2 antibody (ab151293) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS2 antibody (ab151293)Immunohistochemical analysis of paraffin-embedded J5 xenograft labeling BCAS2 with ab151293 antibody at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-BCAS2 antibody (ab151293)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling BCAS2 with ab151293 antibody at 1/200 dilution. Bottom figure represents cells costained with Hoechst 33342.
Protocols
References
This product has been referenced in:
- Lang B & Zhao S miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2. Oncol Rep 39:71-80 (2018). Read more (PubMed: 29115564) »
