Key features and details
- Rabbit polyclonal to BCAS2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BCAS2 antibody
See all BCAS2 primary antibodies
DescriptionRabbit polyclonal to BCAS2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 19-204 of Human BCAS2
- NT2D1 cells; IMR32 cells; MCF-7 cell lysate; HeLa cells; J5 xenograft.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in mRNA splicing.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the SPF27 family.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- bcas2 antibody
- Breast carcinoma amplified sequence 2 antibody
- Breast carcinoma-amplified sequence 2 antibody
Anti-BCAS2 antibody (ab151293) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin-embedded J5 xenograft labeling BCAS2 with ab151293 antibody at 1/100 dilution.
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling BCAS2 with ab151293 antibody at 1/200 dilution. Bottom figure represents cells costained with Hoechst 33342.
ab151293 has been referenced in 1 publication.
- Lang B & Zhao S miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2. Oncol Rep 39:71-80 (2018). PubMed: 29115564