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Anti-BCAS3 antibody (ab71162)

  • Datasheet
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Immunoprecipitation - Anti-BCAS3 antibody (ab71162)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS3 antibody (ab71162)
  • Western blot - Anti-BCAS3 antibody (ab71162)

Key features and details

  • Rabbit polyclonal to BCAS3
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-BCAS3 antibody
    See all BCAS3 primary antibodies
  • Description

    Rabbit polyclonal to BCAS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rhesus monkey, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between residue 870 and the C terminus (residue 928) of human Breast carcinoma amplified sequence 3 (BAB15156.1)

  • Positive control

    • Whole cell lysate from MCF7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cancer
    • Tumor biomarkers
    • Other
    • Stem Cells
    • Cancer Stem Cells
    • Intracellular

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)

Applications

Our Abpromise guarantee covers the use of ab71162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines.
  • Involvement in disease

    Note=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4.
  • Sequence similarities

    Belongs to the WD repeat BCAS3 family.
    Contains 3 WD repeats.
  • Developmental stage

    Fetal.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9H6U6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54828 Human
    • Entrez Gene: 192197 Mouse
    • Omim: 607470 Human
    • SwissProt: Q9H6U6 Human
    • SwissProt: Q8CCN5 Mouse
    • Unigene: 655028 Human
    • Alternative names

      • 1500019F07Rik antibody
      • 2610028P08Rik antibody
      • AU021018 antibody
      • BC028339 antibody
      • BCAS3 antibody
      • BCAS3_HUMAN antibody
      • Breast carcinoma-amplified sequence 3 antibody
      • FLJ20128 antibody
      • GAOB1 antibody
      • K20D4 antibody
      • MAAB antibody
      • Maab1 protein antibody
      • MGC4973 antibody
      • rudhira antibody
      see all

    Images

    • Immunoprecipitation - Anti-BCAS3 antibody (ab71162)
      Immunoprecipitation - Anti-BCAS3 antibody (ab71162)
      Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
      Lane 1: ab71162 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded.
      Detection: Chemiluminescence with an exposure time of 1 second.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS3 antibody (ab71162)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS3 antibody (ab71162)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection: DAB.
    • Western blot - Anti-BCAS3 antibody (ab71162)
      Western blot - Anti-BCAS3 antibody (ab71162)
      All lanes : Anti-BCAS3 antibody (ab71162) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from MCF7 cells at 50 µg
      Lane 2 : Whole cell lysate from MCF7 cells at 15 µg
      Lane 3 : Whole cell lysate from MCF7 cells at 5 µg

      Developed using the ECL technique.

      Predicted band size: 101 kDa
      Observed band size: 117 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab71162? Please let us know so that we can cite the reference in this datasheet.

    ab71162 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunoprecipitation abreview for Anti-BCAS3 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (Melanoma Cell Line)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Protein A
    Specification
    Melanoma Cell Line
    Read More

    John Finnigan

    Verified customer

    Submitted Apr 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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