Anti-BCAS3 antibody (ab71162)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-BCAS3 antibody
See all BCAS3 primary antibodies -
Description
Rabbit polyclonal to BCAS3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rhesus monkey, Orangutan
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Immunogen
A synthetic peptide corresponding to a region between residue 870 and the C terminus (residue 928) of human Breast carcinoma amplified sequence 3 (BAB15156.1)
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Positive control
- Whole cell lysate from MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab71162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines. -
Involvement in disease
Note=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4. -
Sequence similarities
Belongs to the WD repeat BCAS3 family.
Contains 3 WD repeats. -
Developmental stage
Fetal. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 54828 Human
- Entrez Gene: 192197 Mouse
- Omim: 607470 Human
- SwissProt: Q9H6U6 Human
- SwissProt: Q8CCN5 Mouse
- Unigene: 655028 Human
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Alternative names
- 1500019F07Rik antibody
- 2610028P08Rik antibody
- AU021018 antibody
see all
Images
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Immunoprecipitation - Anti-BCAS3 antibody (ab71162)Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
Lane 1: ab71162 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 1 second. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS3 antibody (ab71162)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - Anti-BCAS3 antibody (ab71162)All lanes : Anti-BCAS3 antibody (ab71162) at 0.04 µg/ml
Lane 1 : Whole cell lysate from MCF7 cells at 50 µg
Lane 2 : Whole cell lysate from MCF7 cells at 15 µg
Lane 3 : Whole cell lysate from MCF7 cells at 5 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
References
ab71162 has not yet been referenced specifically in any publications.
