Key features and details
- Rabbit polyclonal to BCAS3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BCAS3 antibody
See all BCAS3 primary antibodies
DescriptionRabbit polyclonal to BCAS3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rhesus monkey, Orangutan
A synthetic peptide corresponding to a region between residue 870 and the C terminus (residue 928) of human Breast carcinoma amplified sequence 3 (BAB15156.1)
- Whole cell lysate from MCF7 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Tissue specificityExpressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines.
Involvement in diseaseNote=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4.
Sequence similaritiesBelongs to the WD repeat BCAS3 family.
Contains 3 WD repeats.
- Information by UniProt
- 1500019F07Rik antibody
- 2610028P08Rik antibody
- AU021018 antibody
Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
Lane 1: ab71162 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 1 second.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-BCAS3 antibody (ab71162) at 0.04 µg/ml
Lane 1 : Whole cell lysate from MCF7 cells at 50 µg
Lane 2 : Whole cell lysate from MCF7 cells at 15 µg
Lane 3 : Whole cell lysate from MCF7 cells at 5 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab71162 has not yet been referenced specifically in any publications.