Overview

  • Product name

  • Description

    Rabbit polyclonal to BCAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C terminal region of Human BCAT1

Properties

Applications

Our Abpromise guarantee covers the use of ab125209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. The detection limit for BCAT 1 is approximately 0.1 ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
  • Tissue specificity

    During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
  • Sequence similarities

    Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCAT antibody
    • BCAT(c) antibody
    • Bcat1 antibody
    • BCAT1_HUMAN antibody
    • BCT1 antibody
    • Branched chain amino acid aminotransferase, cytosolic antibody
    • branched chain amino-acid transaminase 1, cytosolic antibody
    • branched chain aminotransferase 1 antibody
    • branched chain aminotransferase 1, cytosolic antibody
    • Branched-chain-amino-acid aminotransferase antibody
    • cytosolic antibody
    • DKFZp686E12175 antibody
    • EC 2.6.1.42 antibody
    • ECA39 antibody
    • MECA39 antibody
    • placental protein 18 antibody
    • PNAS 121 antibody
    • PP18 antibody
    • Protein ECA39 antibody
    see all

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I am very pleased to hear you would like to accept our offer and testab125209 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ratand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. I have attached an image for IHC-P in human mammary cancer tissue. The homology between the immunogen and the rat tissue is 82%. Generally we predict reactivity at 85% or higher, so there is a definite chance you could be successful with this product.

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