Anti-BCAT1 antibody (ab232700)
Key features and details
- Rabbit polyclonal to BCAT1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-BCAT1 antibody
See all BCAT1 primary antibodies -
Description
Rabbit polyclonal to BCAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse BCAT1 aa 150 to the C-terminus. (Expressed in E.coli).
Database link: P24288 -
Positive control
- WB: Recombinant mouse BCAT1 protein; Mouse cerebrum and kidney lysates; Rat cerebrum lysate. IHC-P: Mouse pancreas and kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232700 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 43 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 43 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. -
Tissue specificity
During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma. -
Sequence similarities
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 12035 Mouse
- Entrez Gene: 29592 Rat
- SwissProt: P24288 Mouse
- SwissProt: P54690 Rat
- Unigene: 4606 Mouse
- Unigene: 8273 Rat
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Alternative names
- BCAT antibody
- BCAT(c) antibody
- Bcat1 antibody
see all
Images
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Anti-BCAT1 antibody (ab232700) at 2 µg/ml + Recombinant mouse BCAT1 protein
Predicted band size: 43 kDa -
Anti-BCAT1 antibody (ab232700) at 2 µg/ml + Mouse cerebrum lysate
Predicted band size: 43 kDa -
Anti-BCAT1 antibody (ab232700) at 2 µg/ml + Mouse kidney lysate
Predicted band size: 43 kDa -
Anti-BCAT1 antibody (ab232700) at 2 µg + Rat cerebrum lysate
Predicted band size: 43 kDa -
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for BCAT1 using ab232700 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse pancreas tissue stained for BCAT1 using ab232700 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab232700 has been referenced in 1 publication.
- Wu Z et al. Exploring the significance of PAK1 through chromosome conformation signatures in ibrutinib-resistant chronic lymphocytic leukaemia. Mol Oncol 16:2920-2935 (2022). PubMed: 35811334