Key features and details
- Rabbit polyclonal to BCAT1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-BCAT1 antibody
See all BCAT1 primary antibodies
DescriptionRabbit polyclonal to BCAT1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Sheep
Recombinant fragment (His-T7-tag) corresponding to Human BCAT1 aa 174-386. (Expressed in E.coli).
GVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKM GGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGE EELATPPLDGIILPGVTRRCILDLAHQWGEFKVSERYLTMDDLTTALEGN RVREMFGSGTACVVCPVSDILYKGETIHIPTMENGPKLASRILSKLTDIQ YGREESDWTIVLS
Database link: P54687
- IHC-P: Human kidney tissue. WB: Recombinant human BCAT1 protein; Rat cerebrum lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232918 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 43 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCatalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Tissue specificityDuring embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
Sequence similaritiesBelongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
- Information by UniProt
- BCAT antibody
- BCAT(c) antibody
- Bcat1 antibody
Anti-BCAT1 antibody (ab232918) at 2 µg/ml + Rat cerebrum lysate
Predicted band size: 43 kDa
Anti-BCAT1 antibody (ab232918) at 2 µg/ml + Recombinant human BCAT1 protein
Predicted band size: 43 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for BCAT1 using ab232918 at 20µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232918 has not yet been referenced specifically in any publications.