Key features and details
- Mouse monoclonal [7G3A11] to BCAT2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-BCAT2 antibody [7G3A11]
See all BCAT2 primary antibodies
DescriptionMouse monoclonal [7G3A11] to BCAT2
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human BCAT2 aa 259-393. Expressed in E.coli.
Database link: O15382-1
- WB: Human BCAT2 recombinant protein; BCAT2-hIgGFc transfected HEK-293 cell lysate. Flow Cytometry: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab233650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|Flow Cyt||1/200 - 1/400.|
FunctionCatalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
Sequence similaritiesBelongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationCytoplasm and Mitochondrion.
- Information by UniProt
- BCAM antibody
- BCAT 2 antibody
- BCAT(m) antibody
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BCAT2 using ab233650 at 1/200 dilution (green) compared to the negative control (red).
Anti-BCAT2 antibody [7G3A11] (ab233650) at 1/500 dilution + Human BCAT2 recombinant protein (amino acids 259-393)
(Expected MW is 41.5 kDa)
All lanes : Anti-BCAT2 antibody [7G3A11] (ab233650) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BCAT2 (amino acids 259-393)-hIgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233650 has not yet been referenced specifically in any publications.