Anti-BCAT2 antibody [7G3A11] (ab233650)
Key features and details
- Mouse monoclonal [7G3A11] to BCAT2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BCAT2 antibody [7G3A11]
See all BCAT2 primary antibodies -
Description
Mouse monoclonal [7G3A11] to BCAT2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BCAT2 aa 259-393. Expressed in E.coli.
Database link: O15382-1 -
Positive control
- WB: Human BCAT2 recombinant protein; BCAT2-hIgGFc transfected HEK-293 cell lysate. Flow Cytometry: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
7G3A11 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 587 Human
- Omim: 113530 Human
- SwissProt: O15382 Human
- Unigene: 512670 Human
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Alternative names
- BCAM antibody
- BCAT 2 antibody
- BCAT(m) antibody
see all
Images
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BCAT2 using ab233650 at 1/200 dilution (green) compared to the negative control (red).
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Anti-BCAT2 antibody [7G3A11] (ab233650) at 1/500 dilution + Human BCAT2 recombinant protein (amino acids 259-393)
(Expected MW is 41.5 kDa)
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All lanes : Anti-BCAT2 antibody [7G3A11] (ab233650) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BCAT2 (amino acids 259-393)-hIgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233650 has not yet been referenced specifically in any publications.