Overview

  • Product name

  • Description

    Rabbit polyclonal to BCAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 25-302 of Human BCAT2 (NP_001181).

  • Positive control

    • Raji and NIH3T3 whole cell lysates; A431 cells; Ca922; H1299, HeLaS3, HepG2, Molt4 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab95976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Cytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCAM antibody
    • BCAT 2 antibody
    • BCAT(m) antibody
    • BCAT2 antibody
    • BCAT2_HUMAN antibody
    • BCATM antibody
    • BCT2 antibody
    • Branched chain aminotransferase 2, mitochondrial antibody
    • Branched-chain-amino-acid aminotransferase antibody
    • ECA40 antibody
    • mitochondrial antibody
    • Placental protein 18 antibody
    • PP18 antibody
    see all

Images

  • Anti-BCAT2 antibody (ab95976) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS-PAGE
  • ab95976, at 1/200 dilution, staining BCAT2 in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
  • ab95976, at 1/100 dilution, staining BCAT2 in paraffin-embedded Ca922 by Immunohistochemistry.
  • Anti-BCAT2 antibody (ab95976) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS-PAGE

References

This product has been referenced in:

  • Singh R  et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). Read more (PubMed: 29618935) »
  • Wang WM  et al. MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway. Front Cell Neurosci 11:96 (2017). WB ; Mouse . Read more (PubMed: 28442995) »
See all 4 Publications for this product

Customer reviews and Q&As

Question
Answer

DISCOUNT CODE: xxxx Expiration date: xxxx I am very pleased to hear you would like to accept our offer and test ab95976 in WB on rat tissues. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for WB on rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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