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  1. Link

    bcat2-antibody-ab95976.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-BCAT2 antibody (ab95976)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (4)

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Western blot - Anti-BCAT2 antibody (ab95976)
  • Immunocytochemistry/ Immunofluorescence - Anti-BCAT2 antibody (ab95976)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (ab95976)
  • Western blot - Anti-BCAT2 antibody (ab95976)

Key features and details

  • Rabbit polyclonal to BCAT2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human BCAT2 protein (ab124554)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-BCAT2 antibody
    See all BCAT2 primary antibodies
  • Description

    Rabbit polyclonal to BCAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 25-302 of Human BCAT2 (NP_001181).

  • Positive control

    • Raji and NIH3T3 whole cell lysates; A431 cells; Ca922; H1299, HeLaS3, HepG2, Molt4 cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • NIH 3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human BCAT2 protein (ab124554)

Applications

Our Abpromise guarantee covers the use of ab95976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Cytoplasm and Mitochondrion.
  • Target information above from: UniProt accession O15382 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 587 Human
    • Entrez Gene: 12036 Mouse
    • Omim: 113530 Human
    • SwissProt: O15382 Human
    • SwissProt: O35855 Mouse
    • Unigene: 512670 Human
    • Unigene: 24210 Mouse
    • Alternative names

      • BCAM antibody
      • BCAT 2 antibody
      • BCAT(m) antibody
      • BCAT2 antibody
      • BCAT2_HUMAN antibody
      • BCATM antibody
      • BCT2 antibody
      • Branched chain aminotransferase 2, mitochondrial antibody
      • Branched-chain-amino-acid aminotransferase antibody
      • ECA40 antibody
      • mitochondrial antibody
      • Placental protein 18 antibody
      • PP18 antibody
      see all

    Images

    • Western blot - Anti-BCAT2 antibody (ab95976)
      Western blot - Anti-BCAT2 antibody (ab95976)
      Anti-BCAT2 antibody (ab95976) at 1/1000 dilution + Raji whole cell lysate at 30 µg

      Predicted band size: 44 kDa



      10% SDS-PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-BCAT2 antibody (ab95976)
      Immunocytochemistry/ Immunofluorescence - Anti-BCAT2 antibody (ab95976)
      ab95976, at 1/200 dilution, staining BCAT2 in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (ab95976)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (ab95976)

      Paraffin embedded Ca922 tissue stained for BCAT2 using ab95976 at 1/100 dilution in immunohistochemical analysis.

       

    • Western blot - Anti-BCAT2 antibody (ab95976)
      Western blot - Anti-BCAT2 antibody (ab95976)
      Anti-BCAT2 antibody (ab95976) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

      Predicted band size: 44 kDa



      10% SDS-PAGE

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab95976? Please let us know so that we can cite the reference in this datasheet.

    ab95976 has been referenced in 4 publications.

    • Singh R  et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). PubMed: 29618935
    • Wang WM  et al. MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway. Front Cell Neurosci 11:96 (2017). WB ; Mouse . PubMed: 28442995
    • Antanaviciute I  et al. Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism. Sci Rep 7:7820 (2017). WB ; Human . PubMed: 28798381
    • Kitsy A  et al. Effects of leucine supplementation and serum withdrawal on branched-chain amino acid pathway gene and protein expression in mouse adipocytes. PLoS One 9:e102615 (2014). WB ; Mouse . PubMed: 25050624

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    Contacted us regarding testing this product on rat tissues.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 23 2012

    Answer

    DISCOUNT CODE: xxxx Expiration date: xxxx I am very pleased to hear you would like to accept our offer and test ab95976 in WB on rat tissues. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for WB on rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jul 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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