Anti-BCAT2 antibody (ab95976)
Key features and details
- Rabbit polyclonal to BCAT2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BCAT2 antibody
See all BCAT2 primary antibodies -
Description
Rabbit polyclonal to BCAT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 25-302 of Human BCAT2 (NP_001181).
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Positive control
- Raji and NIH3T3 whole cell lysates; A431 cells; Ca922; H1299, HeLaS3, HepG2, Molt4 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. | |
IHC-P | 1/100 - 1/500. | |
ICC/IF | 1/100 - 1/200. |
Target
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Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 587 Human
- Entrez Gene: 12036 Mouse
- Omim: 113530 Human
- SwissProt: O15382 Human
- SwissProt: O35855 Mouse
- Unigene: 512670 Human
- Unigene: 24210 Mouse
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Alternative names
- BCAM antibody
- BCAT 2 antibody
- BCAT(m) antibody
see all
Images
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Anti-BCAT2 antibody (ab95976) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS-PAGE -
ab95976, at 1/200 dilution, staining BCAT2 in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT2 antibody (ab95976)
Paraffin embedded Ca922 tissue stained for BCAT2 using ab95976 at 1/100 dilution in immunohistochemical analysis.
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Anti-BCAT2 antibody (ab95976) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS-PAGE
Protocols
References (4)
ab95976 has been referenced in 4 publications.
- Singh R et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). PubMed: 29618935
- Wang WM et al. MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway. Front Cell Neurosci 11:96 (2017). WB ; Mouse . PubMed: 28442995
- Antanaviciute I et al. Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism. Sci Rep 7:7820 (2017). WB ; Human . PubMed: 28798381
- Kitsy A et al. Effects of leucine supplementation and serum withdrawal on branched-chain amino acid pathway gene and protein expression in mouse adipocytes. PLoS One 9:e102615 (2014). WB ; Mouse . PubMed: 25050624