Overview

  • Product name

    Anti-BCAT2 antibody - C-terminal
    See all BCAT2 primary antibodies
  • Description

    Rabbit polyclonal to BCAT2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Human BCAT2 (C terminal). (BC001900). The identity of the protein fusion partner is GST.
    Database link: O15382

  • Positive control

    • Human thyroid cancer tissue; Human placenta tissue, NIH 3T3, Raji and HepG2 lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab197917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/300.
WB 1/200 - 1/1000. Predicted molecular weight: 44 kDa.

Target

  • Function

    Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization

    Cytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCAM antibody
    • BCAT 2 antibody
    • BCAT(m) antibody
    • BCAT2 antibody
    • BCAT2_HUMAN antibody
    • BCATM antibody
    • BCT2 antibody
    • Branched chain aminotransferase 2, mitochondrial antibody
    • Branched-chain-amino-acid aminotransferase antibody
    • ECA40 antibody
    • mitochondrial antibody
    • Placental protein 18 antibody
    • PP18 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling BCAT2 with ab197917 at 1/70 dilution.

  • All lanes : Anti-BCAT2 antibody - C-terminal (ab197917) at 1/312.5 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : NIH 3T3 cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 40 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 5 seconds


    12% SDS-PAGE

References

This product has been referenced in:

  • Dey P  et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature 542:119-123 (2017). IHC-P, IHC ; Human . Read more (PubMed: 28099419) »
See 1 Publication for this product

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