Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 36 kDa.|
FunctionMay promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).
Tissue specificityExpressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen.
Sequence similaritiesBelongs to the BCP1 family.
Developmental stageIsoform 1 is expressed throughout the cell cycle. Isoform 2 is expressed following mitosis and peaks in the G1/S phase of the cell cycle.
Cellular localizationNucleus. Colocalizes with BRCA2 in discrete nuclear foci.
- Information by UniProt
- bccip antibody
- BCCIP_HUMAN antibody
- BCCIPalpha antibody
All lanes : Anti-BCCIP antibody (ab97577) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS Page
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97577 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
ab97577 has not yet been referenced specifically in any publications.