This antibody gave a positive signal in HeLa S3 F1 BCDIN3D Flag whole cell lysate.
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All lanes : Anti-BCDIN3D antibody (ab126589) at 1 µg/ml
Lane 1 : HeLa-S3-F1-BCDIN3D – Flag (positive lysate) Lane 2 : HeLa-S3-F1 (negative lysate)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa Observed band size: 33 kDa Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Lane 2 represents the negative control for BCDIN3D.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab126589 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.