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BCECF-AM, intracellular ratiometric pH indicator (ab143463)

Submit a review Q&A (1)References (2)
Chemical Structure - BCECF-AM, intracellular ratiometric pH indicator (ab143463)

    Key features and details

    • Acetoxymethyl ester of BCECF. Intracellular ratiometric pH indicator.
    • CAS Number: 117464-70-7
    • Purity: > 98%
    • Soluble in DMSO
    • Form / State: Solid
    • Source: Synthetic

    Overview

    • Product name

      BCECF-AM, intracellular ratiometric pH indicator

    • Description

      Acetoxymethyl ester of BCECF. Intracellular ratiometric pH indicator.

    • Purity

      > 98%

    • CAS Number

      117464-70-7

    • Chemical structure

      Chemical Structure

    Properties

    Images

    • Chemical Structure - BCECF-AM, intracellular ratiometric pH indicator (ab143463)
      Chemical Structure - BCECF-AM, intracellular ratiometric pH indicator (ab143463)
      2D chemical structure image of ab143463, BCECF-AM, intracellular ratiometric pH indicator

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab143463? Please let us know so that we can cite the reference in this datasheet.

    ab143463 has been referenced in 2 publications.

    • Lee SP  et al. Characterization of intracellular buffering power in human induced pluripotent stem cells and the loss of pluripotency is delayed by acidic stimulation and increase of NHE1 activity. J Cell Physiol 236:1515-1528 (2021). PubMed: 32841374
    • Buckley BJ  et al. Screening of 5- and 6-Substituted Amiloride Libraries Identifies Dual-uPA/NHE1 Active and Single Target-Selective Inhibitors. Int J Mol Sci 22:N/A (2021). PubMed: 33804289

    Customer reviews and Q&As

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    Question

    Inquiry: Dear Sir or Madam, Could you please let me know if you sell any pH-sensitive fluorophore that could be used in a ratiometric fashion (similar to HPTS), that could be used with linear variation within pH 5-7.5 and could be excited at or above 520 nm? I could not find such a product on your catalogue (the cy5, cy7 dyes being pH-sensitive on-off fluorescence, not ratiometric I believe).

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    Answer

    The only product we have is BCECF-AM Acetoxymethyl ester of BCECF intracellular ratiometric pH indicator, https://www.abcam.com/bcecf-am-ab143463.html.
    The wavelengths given are however different than your requirements; therefore, please have a look at the datasheet and check if it matches your needs.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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