BCECF-AM, intracellular ratiometric pH indicator (ab143463)

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Overview

  • Product name

    BCECF-AM, intracellular ratiometric pH indicator

  • Description

    Acetoxymethyl ester of BCECF. Intracellular ratiometric pH indicator.

  • Biological description

    Acetoxymethyl ester of BCECF. Intracellular ratiometric pH indicator. Converts to fluorescent BCECF (ab120632) via the action of intracellular esterases. Ratio of fluorescence intensities can be converted to pH between 4 and 9. Excitation: 500 nm (high pH), 450 nm (low pH). Emission: 531 nm.

  • Purity

    > 98%

  • CAS Number

    117464-70-7

  • Chemical structure

    Chemical Structure

Properties

References

This product has been referenced in:

  • Ozkan P & Mutharasan R A rapid method for measuring intracellular pH using BCECF-AM. Biochim Biophys Acta 1572:143-8 (2002). Read more (PubMed: 12204343) »
  • Olson DP  et al. Detection of MRP functional activity: calcein AM but not BCECF AM as a Multidrug Resistance-related Protein (MRP1) substrate. Cytometry 46:105-13 (2001). Read more (PubMed: 11309820) »
  • Franck P  et al. Measurement of intracellular pH in cultured cells by flow cytometry with BCECF-AM. J Biotechnol 46:187-95 (1996). Read more (PubMed: 8672290) »

Publishing research using ab143463? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Inquiry: Dear Sir or Madam, Could you please let me know if you sell any pH-sensitive fluorophore that could be used in a ratiometric fashion (similar to HPTS), that could be used with linear variation within pH 5-7.5 and could be excited at or above 520 nm? I could not find such a product on your catalogue (the cy5, cy7 dyes being pH-sensitive on-off fluorescence, not ratiometric I believe).

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Answer

The only product we have is BCECF-AM Acetoxymethyl ester of BCECF intracellular ratiometric pH indicator, https://www.abcam.com/bcecf-am-ab143463.html.
The wavelengths given are however different than your requirements; therefore, please have a look at the datasheet and check if it matches your needs.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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