Key features and details
- Rabbit polyclonal to BCKDHA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BCKDHA antibody
See all BCKDHA primary antibodies
DescriptionRabbit polyclonal to BCKDHA
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 252-432 of Human BCKDHA (UniProt P12694).
- HepG2 whole cell lysate (ab7900); HeLa cells; Human Colon cancer tissue; A549
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionThe branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Involvement in diseaseMaple syrup urine disease 1A
Sequence similaritiesBelongs to the BCKDHA family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
- FLJ45695 antibody
- OVD1A antibody
Anti-BCKDHA antibody (ab126173) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE
ab126173, at 1/500 dilution, staining BCKDHA in methanol-fixed HeLa cells by Immunofluorescence. The lower panel is costained with Hoechst 33342.
ab126173, at 1/250 dilution, staining BCKDHA in paraffin-embedded Human Colon cancer tissue by Immunohistochemistry.
ab126173 has not yet been referenced specifically in any publications.