Product nameAnti-BCKDHA antibody
See all BCKDHA primary antibodies
DescriptionRabbit polyclonal to BCKDHA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Gorilla, Corn
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab138460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThe branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Involvement in diseaseMaple syrup urine disease 1A
Sequence similaritiesBelongs to the BCKDHA family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
- FLJ45695 antibody
- OVD1A antibody
All lanes : Anti-BCKDHA antibody (ab138460) at 1/2000 dilution
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 3 minutes
Immunoprecipitation detection of BCKDHA in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138460 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138460 at 1µg/ml. Chemiluminescence with an exposure time of 30 seconds.
This product has been referenced in:
- Webb LA et al. Changes in tissue abundance and activity of enzymes related to branched-chain amino acid catabolism in dairy cows during early lactation. J Dairy Sci 102:3556-3568 (2019). Read more (PubMed: 30712942) »
- Shao D et al. Glucose promotes cell growth by suppressing branched-chain amino acid degradation. Nat Commun 9:2935 (2018). Read more (PubMed: 30050148) »