Overview

  • Product name

  • Description

    Rabbit polyclonal to BCKDHA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Gorilla, Corn
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 395 and 445 of Human BCKDHA (NP_000700.1).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab138460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease

    Maple syrup urine disease 1A
  • Sequence similarities

    Belongs to the BCKDHA family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
    • FLJ45695 antibody
    • OVD1A antibody
    • 2 oxoisovalerate dehydrogenase (lipoamide) antibody
    • 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
    • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
    • BCKDE1A antibody
    • BCKDH E1 alpha antibody
    • BCKDH E1-alpha antibody
    • BCKDHA antibody
    • Branched chain alpha keto acid dehydrogenase E1 component alpha chain antibody
    • Branched chain keto acid dehydrogenase E1 alpha polypeptide antibody
    • Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease) antibody
    • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain antibody
    • MSU antibody
    • MSUD1 antibody
    • ODBA_HUMAN antibody
    see all

Images

  • All lanes : Anti-BCKDHA antibody (ab138460) at 1/2000 dilution

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation detection of BCKDHA in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138460 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138460 at 1µg/ml. Chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:

  • Webb LA  et al. Changes in tissue abundance and activity of enzymes related to branched-chain amino acid catabolism in dairy cows during early lactation. J Dairy Sci 102:3556-3568 (2019). Read more (PubMed: 30712942) »
  • Shao D  et al. Glucose promotes cell growth by suppressing branched-chain amino acid degradation. Nat Commun 9:2935 (2018). Read more (PubMed: 30050148) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver, skeletal muscle, adipose (AT), mammary)
Gel Running Conditions
Reduced Denaturing
Loading amount
0.25 µg
Specification
Liver, skeletal muscle, adipose (AT), mammary
Blocking step
(agent) for 6 minute(s) · Concentration: 100% · Temperature: RT°C

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Verified customer

Submitted Jun 22 2017

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