Overview

  • Product name

  • Description

    Rabbit polyclonal to BCKDHA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region within N terminal amino acids 72 - 121 (VISGIPIYRV MDRQGQIINP SEDPHLPKEK VLKLYKSMTL LNTMDRILYE) of Human BCKDHA (NP_000700).

  • Positive control

    • MCF7 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by peptide affinity chromatography method.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab90691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling BCKDHA with ab90691 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I and II cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - BCKDHA. Right - Merge.
  • Anti-BCKDHA antibody (ab90691) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 50 kDa



    Gel concentration: 12%

References

This product has been referenced in:

  • Singh R  et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). Read more (PubMed: 29618935) »
  • Kitsy A  et al. Effects of leucine supplementation and serum withdrawal on branched-chain amino acid pathway gene and protein expression in mouse adipocytes. PLoS One 9:e102615 (2014). WB ; Mouse . Read more (PubMed: 25050624) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (L6 myoblasts)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
L6 myoblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Brendan Beatty

Verified customer

Submitted Oct 04 2017

Answer

Thank you for your previous call and for your patience while I have been in touch with my colleagues about ab90691. I apologize for the delay in getting back to you.

At this time, the lower band shown on the Western blot image has not been identified or further chacterized.We believeit is either a degradation product or a nonspecific band.The immunogenic peptide was run through a BLAST search and no potential cross-reactive proteins were identified at that molecular weight.

The blocking peptide for ab90691 is now available as ab134676, and this may help determine specificity of the staining pattern seen in rat brain tissue-

https://www.abcam.com/BCKDHA-peptide-ab134676.html

If you would like to send any images and protocol information, I will see if there are any suggestions to improve the results as well. I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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