Anti-BCKDHA (phospho S293) antibody (ab200577)

Overview

  • Product name

    Anti-BCKDHA (phospho S293) antibody
    See all BCKDHA primary antibodies
  • Description

    Rabbit polyclonal to BCKDHA (phospho S293)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Human, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within BCKDHA (phospho S293). The exact sequence is proprietary.
    Database link: P12694

  • Positive control

    • differentiated NIH3T3-L1 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab200577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-BCKDHA (phospho S293) antibody (ab200577) at 0.4 µg/ml

      Lane 1 : differentiated NIH3T3-L1 whole cell lysate at 50 µg
      Lane 2 : differentiated NIH3T3-L1 whole cell lysate at 15 µg
      Lane 3 : differentiated NIH3T3-L1 whole cell lysate prepared using NETN lysis buffer at 50 µg

      Developed using the ECL technique.

      Predicted band size: 50 kDa


      Exposure time: 30 seconds

    References

    This product has been referenced in:

    • Polakof S  et al. Time-course changes in circulating branched-chain amino acid levels and metabolism in obese Yucatan minipig. Nutrition 50:66-73 (2018). WB . Read more (PubMed: 29547796) »
    • Anderson KA  et al. SIRT4 Is a Lysine Deacylase that Controls Leucine Metabolism and Insulin Secretion. Cell Metab 25:838-855.e15 (2017). Read more (PubMed: 28380376) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Rat Tissue lysate - nuclear (brain)
    Gel Running Conditions
    Reduced Denaturing (NuPAGE® Novex® 4-12% Bis-Tris Protein Gels, 1.0 mm, 12 well)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Sep 16 2015

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (gel 12 %)
    Sample
    Rat Tissue lysate - whole (liver tissue lysate)
    Specification
    liver tissue lysate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Mar 04 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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