Product nameAnti-BCKDHA (phospho S293) antibody
See all BCKDHA primary antibodies
DescriptionRabbit polyclonal to BCKDHA (phospho S293)
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Human, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within BCKDHA (phospho S293). The exact sequence is proprietary.
Database link: P12694
- differentiated NIH3T3-L1 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab200577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.|
FunctionThe branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Involvement in diseaseMaple syrup urine disease 1A
Sequence similaritiesBelongs to the BCKDHA family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
- FLJ45695 antibody
- OVD1A antibody
All lanes : Anti-BCKDHA (phospho S293) antibody (ab200577) at 0.4 µg/ml
Lane 1 : differentiated NIH3T3-L1 whole cell lysate at 50 µg
Lane 2 : differentiated NIH3T3-L1 whole cell lysate at 15 µg
Lane 3 : differentiated NIH3T3-L1 whole cell lysate prepared using NETN lysis buffer at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 30 seconds
This product has been referenced in:
- Polakof S et al. Time-course changes in circulating branched-chain amino acid levels and metabolism in obese Yucatan minipig. Nutrition 50:66-73 (2018). WB . Read more (PubMed: 29547796) »
- Anderson KA et al. SIRT4 Is a Lysine Deacylase that Controls Leucine Metabolism and Insulin Secretion. Cell Metab 25:838-855.e15 (2017). Read more (PubMed: 28380376) »