Overview

  • Product name

  • Description

    Rabbit polyclonal to BCKDK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to Human BCKDK aa 77-367.
    Database link: O14874

  • Positive control

    • WB: HeLaS3, Raji cell lysates; Rat kidney and brain extracts; Mouse brain lysate. IHC-P: SW480 xenograft
  • General notes

     This product was previously labelled as BCKDH kinase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab151297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody
    • [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody
    • BCKD kinase antibody
    • BCKD-kinase antibody
    • BCKD_HUMAN antibody
    • BCKDHKIN antibody
    • Bckdk antibody
    • BCKDKD antibody
    • BDK antibody
    • Branched chain alpha keto acid dehydrogenase kinase antibody
    • Branched chain ketoacid dehydrogenase kinase antibody
    • Branched-chain alpha-ketoacid dehydrogenase kinase antibody
    • mitochondrial antibody
    see all

Images

  • Anti-BCKDK antibody (ab151297) at 1/10000 dilution + Rat kidney extract at 50 µg

    Predicted band size: 46 kDa

  • Anti-BCKDK antibody (ab151297) at 1/10000 dilution + Rat brain extract at 50 µg

    Predicted band size: 46 kDa

  • All lanes : Anti-BCKDK antibody (ab151297) at 1/500 dilution

    Lane 1 : HeLa S3 whole cells lysate
    Lane 2 : Raji whole cells lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Anti-BCKDK antibody (ab151297) at 1/10000 dilution + Mouse brain whole cell lysate at 20 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling BCKDK with ab151297 antibody at 1/100 dilution.

References

ab151297 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 4-12% Bis-Tris Protein Gels, 1.0 mm, 12 well)
Loading amount
30 µg
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 29 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (liver, brain)
Gel Running Conditions
Reduced Denaturing (gel 12 %)
Loading amount
50 µg
Specification
liver, brain
Blocking step
Slim Fast as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 23 2015

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