Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)

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Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
  • Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
  • Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
  • Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
  • OI-RD Scanning - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
  • Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7564] to BCKDK - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BCKDK antibody [EPR7564] - BSA and Azide free
    See all BCKDK primary antibodies

  • Description

    Rabbit monoclonal [EPR7564] to BCKDK - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248230 is the carrier-free version of ab128935 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248230 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BCKDH kinase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • Function

    Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.

  • Post-translational
    modifications

    Autophosphorylated.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody
    • [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody
    • BCKD kinase antibody
    • BCKD-kinase antibody
    • BCKD_HUMAN antibody
    • BCKDHKIN antibody
    • Bckdk antibody
    • BCKDKD antibody
    • BDK antibody
    • Branched chain alpha keto acid dehydrogenase kinase antibody
    • Branched chain ketoacid dehydrogenase kinase antibody
    • Branched-chain alpha-ketoacid dehydrogenase kinase antibody
    • mitochondrial antibody
    see all

Images

  • Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)

    This data was developed using ab128935, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: BCKDH kinase knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab128935 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab128935 was shown to specifically react with BCKDH kinase when BCKDH kinase knockout samples were used. Wild-type and BCKDH kinase knockout samples were subjected to SDS-PAGE. ab128935 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    Anti-BCKDK antibody [EPR7564] (ab128935) at 1/1000 dilution (Purified) + PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    This data was developed using ab128935, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    All lanes : Anti-BCKDK antibody [EPR7564] (ab128935) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    This data was developed using ab128935, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    Western blot - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    All lanes : Anti-BCKDK antibody [EPR7564] (ab128935) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal kidney lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    This data was developed using ab128935, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    OI-RD Scanning - Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    This data was developed using ab128935, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)
    Anti-BCKDK antibody [EPR7564] - BSA and Azide free (ab248230)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab248230? Please let us know so that we can cite the reference in this datasheet.

ab248230 has not yet been referenced specifically in any publications.

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