Overview

  • Product name

  • Description

    Rabbit polyclonal to Bcl-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Bcl-2. (Near N terminal).
    Database link: P10415

  • Positive control

    • ICC/IF: A549 cells. IHC-P: Rat kidney and lung tissues; Mouse lung tissue; Human liver cancer tissue; Breast cancer tissue. WB: HL-60 and BT474 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab196495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/2000. Predicted molecular weight: 26 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • Tissue specificity

    Expressed in a variety of tissues.
  • Involvement in disease

    A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • Post-translational
    modifications

    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • Cellular localization

    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis regulator Bcl 2 antibody
    • Apoptosis regulator Bcl-2 antibody
    • Apoptosis regulator Bcl2 antibody
    • AW986256 antibody
    • B cell CLL/lymphoma 2 antibody
    • B cell leukemia/lymphoma 2 antibody
    • Bcl-2 antibody
    • Bcl2 antibody
    • BCL2_HUMAN antibody
    • C430015F12Rik antibody
    • D630044D05Rik antibody
    • D830018M01Rik antibody
    • Leukemia/lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    • PPP1R50 antibody
    • Protein phosphatase 1 regulatory subunit 50 antibody
    • Protein phosphatase 1, regulatory subunit 50 antibody
    see all

Images

  • All lanes : Anti-Bcl-2 antibody (ab196495) at 1/500 dilution

    Lane 1 : HL-60 cell extract
    Lane 2 : BT474 cell extract

    Predicted band size: 26 kDa

  • Immunofluorescent analysis of A549 (human lung carcinoma cell line) cells labeling Bcl-2 with ab196495 at 1/50 dilution. Blue: DAPI for nuclear staining.

  • Immunohistochemical analysis of paraffin-embedded breast cancer tissue labeling Bcl-2 with ab196495 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling Bcl-2 with ab196495 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Bcl-2 with ab196495 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Bcl-2 with ab196495 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Bcl-2 with ab196495 at 1/100 dilution.

  • All lanes : Anti-Bcl-2 antibody (ab196495)

    Lane 1 : Total protein isolated from brain of rat offspring raised on control mothers
    Lane 2 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin
    Lane 3 : Total protein isolated from brain of rat offspring exposed post natally to 10 mg/kg of cypermethrin
    Lane 4 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin and subsequently rechallenged with cypermethrin (10 mg/kg) at adulthood

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 26 kDa

References

This product has been referenced in:

  • Yue H  et al. Metformin protects against sevoflurane-induced neuronal apoptosis through the S1P1 and ERK signaling pathways. Exp Ther Med 17:1463-1469 (2019). Read more (PubMed: 30680029) »
  • Xiong Y  et al. ERK5-regulated RERG expression promotes cancer progression in prostatic carcinoma. Oncol Rep 41:1160-1168 (2019). Read more (PubMed: 30431143) »
See all 89 Publications for this product

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