Key features and details
- Rabbit polyclonal to Bcl-2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Bcl-2 antibody
See all Bcl-2 primary antibodies
DescriptionRabbit polyclonal to Bcl-2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human Bcl-2. (Near N terminal).
Database link: P10415
- ICC/IF: A549 cells. IHC-P: Rat kidney and lung tissues; Mouse lung tissue; Human liver cancer tissue; Breast cancer tissue. WB: HL-60 and BT474 cell extracts.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 26 kDa.
Primary antibody incubation at 4°C overnight.
|ICC/IF||1/50 - 1/200.|
FunctionSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Tissue specificityExpressed in a variety of tissues.
Involvement in diseaseA chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainBH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
Cellular localizationMitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
All lanes : Anti-Bcl-2 antibody (ab196495) at 1/500 dilution
Lane 1 : HL-60 cell extract
Lane 2 : BT474 cell extract
Predicted band size: 26 kDa
Immunofluorescent analysis of A549 (human lung carcinoma cell line) cells labeling Bcl-2 with ab196495 at 1/50 dilution. Blue: DAPI for nuclear staining.
Immunohistochemical analysis of paraffin-embedded breast cancer tissue labeling Bcl-2 with ab196495 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Bcl-2 with ab196495 at 1/100 dilution.
All lanes : Anti-Bcl-2 antibody (ab196495)
Lane 1 : Total protein isolated from brain of rat offspring raised on control mothers
Lane 2 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin
Lane 3 : Total protein isolated from brain of rat offspring exposed post natally to 10 mg/kg of cypermethrin
Lane 4 : Total protein isolated from brain of rat offspring exposed prenatally to 5 mg/kg of cypermethrin and subsequently rechallenged with cypermethrin (10 mg/kg) at adulthood
Lysates/proteins at 50 µg per lane.
Predicted band size: 26 kDa
ab196495 has been referenced in 177 publications.
- Li J et al. Effect of SOD2 methylation on mitochondrial DNA4834-bp deletion mutation in marginal cells under oxidative stress. Bosn J Basic Med Sci 20:70-77 (2020). PubMed: 31465718
- Yang Z et al. Exosomes derived from cancer stem cells of gemcitabine-resistant pancreatic cancer cells enhance drug resistance by delivering miR-210. Cell Oncol (Dordr) 43:123-136 (2020). PubMed: 31713003
- Liu J et al. The inhibition of miR-101a-3p alleviates H/R injury in H9C2 cells by regulating the JAK2/STAT3 pathway. Mol Med Rep 21:89-96 (2020). PubMed: 31746349
- Wang X & Zhao J Neuroprotective effect of CPCGI on Alzheimer's disease and its mechanism. Mol Med Rep 21:115-122 (2020). PubMed: 31939621
- Liu J et al. The Lipoxin A4 Receptor Agonist BML-111 Alleviates Inflammatory Injury and Oxidative Stress in Spinal Cord Injury. Med Sci Monit 26:e919883 (2020). PubMed: 31971927