• Product name
  • Description
    Rabbit polyclonal to Bcl-2
  • Host species
  • Specificity
    ab59348 detects endogenous levels of total Bcl-2 protein.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Bcl-2.
    Database link: P10415

  • Positive control
    • WB: K562 whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HepG2 cells.
  • General notes

    Store at -20°C for year



Our Abpromise guarantee covers the use of ab59348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.


  • Function
    Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • Tissue specificity
    Expressed in a variety of tissues.
  • Involvement in disease
    A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • Post-translational
    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • Cellular localization
    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl 2 antibody
    • Apoptosis regulator Bcl-2 antibody
    • Apoptosis regulator Bcl2 antibody
    • AW986256 antibody
    • B cell CLL/lymphoma 2 antibody
    • B cell leukemia/lymphoma 2 antibody
    • Bcl-2 antibody
    • Bcl2 antibody
    • BCL2_HUMAN antibody
    • C430015F12Rik antibody
    • D630044D05Rik antibody
    • D830018M01Rik antibody
    • Leukemia/lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    • PPP1R50 antibody
    • Protein phosphatase 1, regulatory subunit 50 antibody
    see all


  • All lanes : Anti-Bcl-2 antibody (ab59348) at 1/500 dilution

    Lane 1 : Extracts from K562 cells with no immunizing peptide
    Lane 2 : Extracts from K562 cells with immunizing peptide

    Observed band size: 26 kDa (why is the actual band size different from the predicted?)

  • ab59348, at a 1/50 dilution, staining human Bcl-2 in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).

  • ELISA analysis of primary cultured rat cortical neurons, detecting Bcl-2 using ab59348.

    Cells were treated with (Tat) or without Tat (control) and either Estradiol, Genistein or Daidzein. Lysates were prepared after 24 hours. A 96-well plate was coated using carbonate coating buffer. 20 µg cell lysate was added to wells and incubated overnight at 4°C. Plates were blocked with 1% BSA for 2 hr at room temperature. Primary antibody (1/5000) was added to sample wells before incubating overnight at 4°C. A goat anti-rabbit ALP-conjugated IgG was used as secondary antibody and phosphatase substrate mixture was added. Absorbance was read at 650nm.

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Bcl-2 with ab59348 in the absence (left image) and presence of the immunizing peptide (right image).


This product has been referenced in:
  • Fu F  et al. NDUFA4 enhances neuron growth by triggering growth factors and inhibiting neuron apoptosis through Bcl-2 and cytochrome C mediated signaling pathway. Am J Transl Res 10:164-174 (2018). Read more (PubMed: 29423002) »
  • Yuan J  et al. Autophagy regulates the degeneration of the auditory cortex through the AMPK-mTOR-ULK1 signaling pathway. Int J Mol Med 41:2086-2098 (2018). Read more (PubMed: 29344647) »

See all 74 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Human Tissue lysate - whole (Heart)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 19 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Normal breast tissue)
Normal breast tissue
Antigen retrieval step
Heat mediated
Blocking step
commercially available kit as blocking agent for 15 minute(s) · Concentration: 100µg/mL · Temperature: 37°C


Verified customer

Submitted Jun 13 2012

Thank you for your reply.

Abreviews for ab32042 Anti-active Caspase 3 antibody [E83-77] and ab59348 Anti-Bcl2 antibody can be submitted via the website. Submitting your protocol and data using our Abreview system will take about 10 minutes. ...

Read More

ab1998 : https://www.abcam.com/ab1998, where the immunogen is described asa peptide corresponding to amino acids 517 to 531 of human AIF. This sequence is identical tothose of mouse and rat AIF.

ab32042 : https://www.abcam.com/ab320...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (Mouse embryonic stem cells or embroid bodies)
Loading amount
50 µg
Mouse embryonic stem cells or embroid bodies
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 09 2008


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