Recombinant Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)](https://www.abcam.com/ps/products/238/ab238042/Images/ab238042-343128-bcl2antibodybcl21878r-ihc-spleen-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BCL2/1878R] to Bcl-2
- Suitable for: IHC-P, WB, Protein Array
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Bcl-2 antibody [BCL2/1878R]
See all Bcl-2 primary antibodies -
Description
Rabbit monoclonal [BCL2/1878R] to Bcl-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Bcl-2.
Database link: P10415 -
Positive control
- IHC-P: Human spleen tissue. WB: MCF7 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
BCL2/1878R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 mins at RT. |
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa.
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| Protein Array |
Use at an assay dependent concentration.
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| Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 mins at RT. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
|
Protein Array
Use at an assay dependent concentration. |
Target
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Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). -
Tissue specificity
Expressed in a variety of tissues. -
Involvement in disease
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. -
Post-translational
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. -
Cellular localization
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 596 Human
- Omim: 151430 Human
- SwissProt: P10415 Human
- Unigene: 150749 Human
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Alternative names
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Bcl-2 using ab238042 at 1 µg/mL in immunohistochemical analysis.
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![Western blot - Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)](https://www.abcam.com/ps/products/238/ab238042/Images/ab238042-343130-bcl2antibodybcl21878r-wb-mcf7-human.jpg)
Western blot - Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)Anti-Bcl-2 antibody [BCL2/1878R] (ab238042) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 26 kDa -
Protein Array - Anti-Bcl-2 antibody (ab238042)ab238042 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)](https://www.abcam.com/ps/products/238/ab238042/Images/ab238042-4-benefits-of-recombinant-antibodies.png)
Anti-Bcl-2 antibody [BCL2/1878R] (ab238042)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab238042 has been referenced in 1 publication.
- Si H et al. Anti-Tumor Effect of Celastrol on Hepatocellular Carcinoma by the circ_SLIT3/miR-223-3p/CXCR4 Axis. Cancer Manag Res 13:1099-1111 (2021). PubMed: 33574707
