Immunocytochemistry/ Immunofluorescence abreview for Anti-Bcl-2 antibody [E17]

Below Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were MeOH-fixed, immunofluorescently labelled and counterstained with DAPI. Under these conditions, this antibody produces a weak staining pattern that does not appear specific – there is no nuclear enrichment and the mitochondrial association is not readily apparent. Paraformaldehyde fixed samples do not give the expected staining pattern, rather they produce a nucleolar enriched staining pattern.

Dr. Kirk Mcmanus

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Submitted Dec 08 2017

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