Asynchronous cells were MeOH-fixed, immunofluorescently labelled and counterstained with DAPI. Under these conditions, this antibody produces a weak staining pattern that does not appear specific – there is no nuclear enrichment and the mitochondrial association is not readily apparent. Paraformaldehyde fixed samples do not give the expected staining pattern, rather they produce a nucleolar enriched staining pattern.
Dr. Kirk Mcmanus
Submitted Dec 08 2017
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