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  1. Link

    bcl-2-antibody-epr17509-ab182858.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Bcl-2 antibody [EPR17509] (ab182858)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (13) Submit a question References (288)

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Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
  • Anti-Bcl-2 antibody [EPR17509] (ab182858)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17509] to Bcl-2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

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Overview

  • Product name

    Anti-Bcl-2 antibody [EPR17509]
    See all Bcl-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR17509] to Bcl-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human tonsil and thymus lysates; Jurkat, U-937, THP-1, HeLa, C2C12, WEHI -3 and NIH/3T3 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Human fetal kidney and fetal spleen lysates; Wild-type Hap1 cell lysate. IHC-P: Human tonsil tissue, Human endometrial cancer tissue, Mouse spleen tissue. Flow Cyt (intra): Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17509
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • HRP Anti-Bcl-2 antibody [EPR17509] (ab209039)
    • Anti-Bcl-2 antibody [EPR17509] - BSA and Azide free (ab219608)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/250.
WB (5)
1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P (7)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/250.
WB
1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • Tissue specificity

    Expressed in a variety of tissues.
  • Involvement in disease

    A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • Post-translational
    modifications

    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • Cellular localization

    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P10415 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 596 Human
    • Entrez Gene: 12043 Mouse
    • Omim: 151430 Human
    • SwissProt: P10415 Human
    • SwissProt: P10417 Mouse
    • Unigene: 150749 Human
    • Unigene: 257460 Mouse
    • Alternative names

      • Apoptosis regulator Bcl 2 antibody
      • Apoptosis regulator Bcl-2 antibody
      • Apoptosis regulator Bcl2 antibody
      • AW986256 antibody
      • B cell CLL/lymphoma 2 antibody
      • B cell leukemia/lymphoma 2 antibody
      • Bcl-2 antibody
      • Bcl2 antibody
      • BCL2_HUMAN antibody
      • C430015F12Rik antibody
      • D630044D05Rik antibody
      • D830018M01Rik antibody
      • Leukemia/lymphoma, B-cell, 2 antibody
      • Oncogene B-cell leukemia 2 antibody
      • PPP1R50 antibody
      • Protein phosphatase 1 regulatory subunit 50 antibody
      • Protein phosphatase 1, regulatory subunit 50 antibody
      see all

    Images

    • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      All lanes : Anti-Bcl-2 antibody [EPR17509] (ab182858) at 1/2000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : BCL2 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 26 kDa
      Observed band size: 26 kDa



      Lanes 1- 2: Merged signal (red and green). Green - ab182858 observed at 26 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab182858 was shown to react with Bcl-2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255364 (knockout cell lysate ab263752) was used. Wild-type HeLa and BCL2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab182858 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl-2 with ab182858 at 1/1000 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

      Cytoplasm, nuclear membrane and nucleus staining on lymphocytes of Human tonsil tissue is observed.

      Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody followed by ab97051 at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [EPR17509] (ab182858)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Bcl-2 with ab182858 at 1/250 (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue)). Goat anti rabbit IgG (FITC) at 1/500 was used as the secondary antibody. 

       

       

    • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      All lanes : Anti-Bcl-2 antibody [EPR17509] (ab182858) at 1 µg/ml

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : BCL2 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : THP-1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 26 kDa
      Observed band size: 26 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab182858 observed at 26 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab182858 was shown to specifically react with BCL2 when BCL2 knockout samples were used. Wild-type and BCL2 knockout samples were subjected to SDS-PAGE. Ab182858 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      All lanes : Anti-Bcl-2 antibody [EPR17509] (ab182858) at 1/10000 dilution

      Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
      Lane 2 : WEHI-3 (mouse leukemia cell line) whole cell lysate at 20 µg
      Lane 3 : Mouse hippocampus at 10 µg
      Lane 4 : Mouse heart at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa


      Exposure time: 8 seconds


      Blocking/Diluting buffer 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)

      Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Bcl-2 with ab182858 at 1/1000 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

      Cytoplasm, nuclear membrane and nucleus staining on lymphocytes and cancer cells of  Human endometrial cancer  tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by ab97051 at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      All lanes : Anti-Bcl-2 antibody [EPR17509] (ab182858) at 1/20000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human thymus lysate
      Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
      Lane 4 : U-937 (Human histiocytic lymphoma cells) whole cell lysate
      Lane 5 : THP-1 (Human monocytic leukemia cells) whole cell lysate
      Lane 6 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 7 : C2C12 (Mouse myoblast cell line) whole cell lysate
      Lane 8 : WEHI-3 (Mouse leukemia cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 26 kDa


      Exposure time: 1 minute


      Blocking and diluting buffer was 5% NFDM /TBST.

    • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      All lanes : Anti-Bcl-2 antibody [EPR17509] (ab182858) at 1/2000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen lysate
      Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 26 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST.

    • Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Western blot - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      All lanes : Anti-Bcl-2 antibody [EPR17509] (ab182858) at 1/2000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa
      Observed band size: 26 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (ab182858)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Bcl-2 with ab182858 at 1/1000 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

      Cytoplasm, nuclear membrane and nucleus staining on lymphocytes of Mouse spleen tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by ab97051 at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Bcl-2 antibody [EPR17509] (ab182858)
      Anti-Bcl-2 antibody [EPR17509] (ab182858)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (288)

    Publishing research using ab182858? Please let us know so that we can cite the reference in this datasheet.

    ab182858 has been referenced in 288 publications.

    • Chen X  et al. KLF4 downregulates FGF21 to activate inflammatory injury and oxidative stress of LPS-induced ATDC5 cells via SIRT1/NF-?B/p53 signaling. Mol Med Rep 25:N/A (2022). WB ; Human . PubMed: 35293599
    • Xing W  et al. MiR-33a-5p targets NOMO1 to modulate human cardiomyocyte progenitor cells proliferation and differentiation and apoptosis. J Recept Signal Transduct Res 41:476-487 (2021). PubMed: 33054489
    • Liu B  et al. mir-126-5p Promotes Cisplatin Sensitivity of Non-Small-Cell Lung Cancer by Inhibiting ADAM9. Biomed Res Int 2021:6622342 (2021). PubMed: 34055989
    • Luo T  et al. Ginkgolide B-mediated therapeutic effects on perioperative neurocognitive dysfunction are associated with the inhibition of iNOS-mediated production of NO. Mol Med Rep 24:N/A (2021). PubMed: 34080648
    • Xian W  et al. CXCR3 alleviates renal ischemia-reperfusion injury via increase of Tregs. Mol Med Rep 24:N/A (2021). PubMed: 34080653
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    Western blot abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (CT26 cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Tris-Bis gel)
    Loading amount
    40 µg
    Specification
    CT26 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 08 2021

    Western blot abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (primary AML cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Tris-Bis gel)
    Loading amount
    40 µg
    Specification
    primary AML cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 08 2021

    Western blot abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    4mg/ml bevacizumab for 48hrs
    Specification
    Glioblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 09 2020

    Western blot abreview for Anti-Bcl-2 antibody [EPR17509]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    MEFs
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Yonatan Feuermann

    Verified customer

    Submitted Jun 04 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (CT26 tumor taken out Balb/C mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA ph9
    Permeabilization
    No
    Specification
    CT26 tumor taken out Balb/C mice
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 31 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (A549 tumor taken from Balb/c nude mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA ph9
    Permeabilization
    No
    Specification
    A549 tumor taken from Balb/c nude mice
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 31 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT-29 (ATCC® HTB-38™) cells)
    Permeabilization
    Yes - 0.1% triton-X100 in PBS
    Specification
    HT-29 (ATCC® HTB-38™) cells
    Blocking step
    Innovex Background Buster as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 18 2019

    Western blot abreview for Anti-Bcl-2 antibody [EPR17509]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Denaturing (12% gel)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 19 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lymphoma xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    lymphoma xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 14 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bcl-2 antibody [EPR17509]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cat Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 14 2019

    1-10 of 13 Abreviews or Q&A

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