Product nameAnti-bcl-6 antibody [EPR11410-43]
See all bcl-6 primary antibodies
DescriptionRabbit monoclonal [EPR11410-43] to bcl-6
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Recombinant fragment within Human bcl-6 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P41182
- Ramos, Daudi and Raji cell lysates; Human lymph node, tonsil and B cell lymphoma tissues; Ramos cells.
We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab172610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa).|
|IHC-P||1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/200 - 1/500. See IHC antigen retrieval protocols.
- B Cell Lymphoma 6 Protein antibody
- B-cell CLL/lymphoma 6 antibody
- B-cell lymphoma 5 protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node in stomach tissue sections labeling bcl-6 with purified ab172610 at 1:1500 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Anti-bcl-6 antibody [EPR11410-43] (ab172610) at 1/2000 dilution (Purified) + Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 78 kDa
Observed band size: 78 kDa
Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
All lanes : Anti-bcl-6 antibody [EPR11410-43] (ab172610)
Lane 1 : Ramos cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDa
This image was produced using unpurified antibody.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
ab172610 staining bcl-6 in Human tonsil tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with 1% PB ab64226 for 10 minutes at room tempreture and antigen retrieval was by heat mediation in EDTA pH 9. The sample was incubated with primary antibody (1/100 in Leica Bond Diluent) at for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (undiluted) was used as the secondary antibody. This image was produced using unpurified antibody.
Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
ab172610 has been referenced in 3 publications.
- Song Y et al. Increased frequency of thymic T follicular helper cells in myasthenia gravis patients with thymoma. J Thorac Dis 8:314-22 (2016). IHC ; Human . PubMed: 27076925
- Sun Y et al. MicroRNA-155 Promotes the Directional Migration of Resident Smooth Muscle Progenitor Cells by Regulating Monocyte Chemoattractant Protein 1 in Transplant Arteriosclerosis. Arterioscler Thromb Vasc Biol 36:1230-9 (2016). PubMed: 27079875
- Meyerholz DK et al. Immunohistochemical Detection of Markers for Translational Studies of Lung Disease in Pigs and Humans. Toxicol Pathol 44:434-41 (2016). IHC-P ; Pig . PubMed: 26511846