Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [15C] to Bcl 7A - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-Bcl 7A antibody [15C] - BSA and Azide free
See all Bcl 7A primary antibodies
DescriptionMouse monoclonal [15C] to Bcl 7A - BSA and Azide free
Tested applicationsSuitable for: IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IP or WB
Species reactivityReacts with: Human
Recombinant fragment. This information is considered to be commercially sensitive.
- IHC-P: Human tonsil tissue.
ab252800 is a PBS only version of ab241550.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab252800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.385 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Involvement in diseaseNote=Chromosomal aberrations involving BCL7A may be a cause of B-cell non-Hodgkin lymphoma. Three-way translocation t(8;14;12)(q24.1;q32.3;q24.1) with MYC and with immunoglobulin gene regions.
Sequence similaritiesBelongs to the BCL7 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- B cell CLL/lymphoma 7 antibody
- B cell CLL/lymphoma 7 protein family member A antibody
- B cell CLL/lymphoma 7A antibody
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl 7A with ab241550 at 2.385 µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on the tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
The section was incubated with ab241550 for 30 mins at room temperature.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241550).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252800 has not yet been referenced specifically in any publications.