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Cell Biology Apoptosis Intracellular Bcl2 Family
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Bcl G/BCL2L14 overexpression 293T lysate (whole cell) (ab94328)

  • Datasheet
  • SDS
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SDS-PAGE - <p>Bcl G/BCL2L14 overexpression 293T lysate (whole cell)</p> (ab94328)
  • Western blot - <p>Bcl G/BCL2L14 overexpression 293T lysate (whole cell)</p> (ab94328)

Overview

  • Product name

    Bcl G/BCL2L14 overexpression 293T lysate (whole cell)
  • General notes

    ab94328 is a 293T cell transfected lysate in which Human Bcl G/BCL2L14 has been transiently over-expressed using a pCMV-Bcl G/BCL2L14 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Tags & Cell Markers
    • Cell Type Markers
    • Apoptotic
  • Background

    Bcl-G is a novel pro-apoptotic member of the Bcl-2 family. The human Bcl-G gene encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L).

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab94328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution.
Notes
WB
Use at an assay dependent dilution.

Images

  • SDS-PAGE - <p>Bcl G/BCL2L14 overexpression 293T lysate (whole cell)</p> (ab94328)
    SDS-PAGE -

    Bcl G/BCL2L14 overexpression 293T lysate (whole cell)

    (ab94328)
    ab94328 at 15µg/lane on an SDS-PAGE gel.
  • Western blot - <p>Bcl G/BCL2L14 overexpression 293T lysate (whole cell)</p> (ab94328)
    Western blot -

    Bcl G/BCL2L14 overexpression 293T lysate (whole cell)

    (ab94328)
    All lanes : Anti-Bcl G/BCL2L14 antibody (ab57742) at 1/500 dilution

    Lane 1 : Bcl G/BCL2L14 overexpression 293T lysate (whole cell) (ab94328)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Protocols

  • Transfected Lysate Preparation Notes

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab94328? Please let us know so that we can cite the reference in this datasheet.

ab94328 has not yet been referenced specifically in any publications.

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