Bcl G/BCL2L14 overexpression 293T lysate (whole cell) (ab94328)
- Datasheet
- References
- Protocols
Overview
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Product name
Bcl G/BCL2L14 overexpression 293T lysate (whole cell) -
Description
Bcl G/BCL2L14 overexpression lysate -
General notes
ab94328 is a 293T cell transfected lysate in which Human Bcl G/BCL2L14 has been transiently over-expressed using a pCMV-Bcl G/BCL2L14 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol -
Concentration information loading... -
Research areas
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Background
Bcl-G is a novel pro-apoptotic member of the Bcl-2 family. The human Bcl-G gene encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L).
Applications
Our Abpromise guarantee covers the use of ab94328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. |
Images
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SDS-PAGE -Bcl G/BCL2L14 overexpression 293T lysate (whole cell)
(ab94328)ab94328 at 15µg/lane on an SDS-PAGE gel. -
Western blot -Bcl G/BCL2L14 overexpression 293T lysate (whole cell)
(ab94328)All lanes : Anti-Bcl G/BCL2L14 antibody (ab57742) at 1/500 dilution
Lane 1 :Bcl G/BCL2L14 overexpression 293T lysate (whole cell) (ab94328)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Protocols
References
ab94328 has not yet been referenced specifically in any publications.
