• Product name
    Anti-Bcl-XL antibody [7B2.5] (FITC)
    See all Bcl-XL primary antibodies
  • Description
    Mouse monoclonal [7B2.5] to Bcl-XL (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Bcl-XL.
    Database link: Q07817


Our Abpromise guarantee covers the use of ab26148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab91539 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.


  • Function
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificity
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localization
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all


  • Flow Cytometry analysis of T cell leukemia cell line Jurkat intracellularly stained with Mouse Anti-Human Bcl-xL-FITC (ab26148) 1 μg/106 cells (purple). Grey - Isotype Control, Mouse IgG3-FITC.

  • Overlay histogram showing DU145 cells stained with ab26148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26148, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Chong HZ  et al. In vitro evaluation of Pandanus amaryllifolius ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis. BMC Complement Altern Med 12:134 (2012). Flow Cyt . Read more (PubMed: 22909149) »
  • Chong HZ  et al. In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. BMC Complement Altern Med 12:35 (2012). Flow Cyt . Read more (PubMed: 22471785) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for your enquiry. I am looking into your question about the intensity of the antibody relative to unstained cells, and will get back to you once I have further information. As to your other question, interestingly enough I was discussing this very question with a colleague today, and in short, yes. The signal from PE is brighter than that of FITC, and FITC may not be a good choice if the target you are looking at has low expression levels in the cell. I hope this information helps, I will be in contact with more information when I receive it.

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Thank you for your enquiry. I have not heard of a difference between, for example, PE or FITC conjugated antibodies for intracellular staining in FACS. I consulted with several colleagues as well as searched the web and other antibody company's websites and I cannot find a difference. Yes, FITC = 389 Da, PE = 250 kDa, Antibody = 150 kDa, however, both PE and FITC conjugated antibodies are widely used in FACS. I cannot recommend that either is better than the other. I have heard that Alexa Fluor dyes might have greater photostability and increased fluorescence but I do not know that this has be unequivocally proven. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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