Product nameAnti-Bcl-XL antibody
See all Bcl-XL primary antibodies
DescriptionRabbit polyclonal to Bcl-XL
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cat, Dog, Pig
Synthetic peptide corresponding to Bcl-XL.
Database link: Q07817
- Hodgkins lymphoma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab15274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||1/200. for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.|
FunctionPotent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Tissue specificityBcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainThe BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
modificationsProteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Cellular localizationMitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
- Information by UniProt
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
This product has been referenced in:
- Shen J et al. Lycorine inhibits glioblastoma multiforme growth through EGFR suppression. J Exp Clin Cancer Res 37:157 (2018). Read more (PubMed: 30016965) »
- Zhang K et al. Bcl-xL overexpression and its association with the progress of tongue carcinoma. Int J Clin Exp Pathol 7:7360-77 (2014). Read more (PubMed: 25550772) »