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    bcl-xl-antibody-ab45002.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Anti-Bcl-XL antibody (ab45002)

  • Datasheet
  • SDS
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Western blot - Anti-Bcl-XL antibody (ab45002)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (ab45002)

Key features and details

  • Rabbit polyclonal to Bcl-XL
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Primary
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Anti-Bcl-XL antibody [EPR16642] (ab178844)
Protein
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Recombinant Human Bcl-XL protein (His tag) (ab180062)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Bcl-XL antibody
    See all Bcl-XL primary antibodies
  • Description

    Rabbit polyclonal to Bcl-XL
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Bcl-XL aa 3-14 (N terminal).
    Sequence:

    C-QSNRELVVDFLS


    Database link: Q07817
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Hodgkin's lymphoma.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 0.01% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Bcl-XL protein (His tag) (ab180062)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Sheep
Rabbit
Cat
Dog
Pig
Application Abreviews Notes
IHC-P
1/2000.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
Notes
IHC-P
1/2000.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificity

    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    modifications

    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localization

    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Target information above from: UniProt accession Q07817 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 598 Human
    • Entrez Gene: 397536 Pig
    • Omim: 600039 Human
    • SwissProt: Q07817 Human
    • SwissProt: O77737 Pig
    • Unigene: 516966 Human
    • Alternative names

      • Apoptosis regulator Bcl X antibody
      • Apoptosis regulator Bcl-X antibody
      • Apoptosis regulator BclX antibody
      • B cell lymphoma 2 like antibody
      • B2CL1_HUMAN antibody
      • Bcl 2 like 1 protein antibody
      • Bcl X antibody
      • Bcl xL antibody
      • BCL XL/S antibody
      • Bcl xS antibody
      • Bcl-2-like protein 1 antibody
      • Bcl2 Like 1 antibody
      • Bcl2 related gene antibody
      • Bcl2-L-1 antibody
      • BCL2L antibody
      • Bcl2l1 antibody
      • BCLX antibody
      • BclXL antibody
      • BclXs antibody
      • DKFZp781P2092 antibody
      • PPP1R52 antibody
      • Protein phosphatase 1 regulatory subunit 52 antibody
      see all

    Images

    • Western blot - Anti-Bcl-XL antibody (ab45002)
      Western blot - Anti-Bcl-XL antibody (ab45002)
      All lanes : Anti-Bcl-XL antibody (ab45002) at 1 µg/ml

      Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated
      Lane 2 : Hela Whole Cell Lysate - Staurosporine Treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 26 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 150 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (ab45002)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (ab45002)
      Human normal kidney cortex. Staining is localised to mitochondrial membranes and perinuclear envelope. Left panel: with primary antibody at 4 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplificatio

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab45002? Please let us know so that we can cite the reference in this datasheet.

    ab45002 has been referenced in 1 publication.

    • Hilbe M  et al. Apoptosis in Bovine Viral Diarrhea Virus (BVDV)-Induced Mucosal Disease Lesions: A Histological, Immunohistological, and Virological Investigation. Vet Pathol : (2012). IHC-P ; Cow . PubMed: 22700847

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