Overview

  • Product name
    Anti-Bcl-XL antibody
    See all Bcl-XL primary antibodies
  • Description
    Rabbit polyclonal to Bcl-XL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Sheep, Rabbit, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Bcl-XL aa 3-14 (N terminal).
    Sequence:

    C-QSNRELVVDFLS


    Database link: Q07817

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Hodgkin's lymphoma.

Applications

Our Abpromise guarantee covers the use of ab45002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

Target

  • Function
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificity
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localization
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Images

  • Western blot - Anti-Bcl-XL antibody (ab45002)
    Western blot - Anti-Bcl-XL antibody (ab45002)
    All lanes : Anti-Bcl-XL antibody (ab45002) at 1 µg/ml

    Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated
    Lane 2 : Hela Whole Cell Lysate - Staurosporine Treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 26 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 100 kDa, 150 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (ab45002)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody (ab45002)
    Human normal kidney cortex. Staining is localised to mitochondrial membranes and perinuclear envelope. Left panel: with primary antibody at 4 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplificatio

References

This product has been referenced in:
  • Hilbe M  et al. Apoptosis in Bovine Viral Diarrhea Virus (BVDV)-Induced Mucosal Disease Lesions: A Histological, Immunohistological, and Virological Investigation. Vet Pathol : (2012). IHC-P ; Cow . Read more (PubMed: 22700847) »

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