Anti-Bcl-XL antibody (ab98143)
Key features and details
- Rabbit polyclonal to Bcl-XL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Bcl-XL antibody
See all Bcl-XL primary antibodies -
Description
Rabbit polyclonal to Bcl-XL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Cat, Pig -
Immunogen
Recombinant fragment corresponding to Human Bcl-XL aa 1-200.
Database link: Q07817-1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
|
IHC-P | (1) |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. -
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. -
Post-translational
modificationsProteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. -
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope. - Information by UniProt
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Database links
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 397536 Pig
- Entrez Gene: 24888 Rat
- Omim: 600039 Human
- SwissProt: Q07817 Human
- SwissProt: Q64373 Mouse
- SwissProt: O77737 Pig
see all -
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Images
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Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunohistochemical analysis of Bcl x in paraffin embedded SW480 xenograft, using ab98143 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab98143 has been referenced in 5 publications.
- Kim HJ et al. Harnessing adipose-derived stem cells to release specialized secretome for the treatment of hepatitis B. Int J Mol Med 47:N/A (2021). PubMed: 33448314
- Li X et al. Knockdown of lncRNA CCAT1 enhances sensitivity of paclitaxel in prostate cancer via regulating miR-24-3p and FSCN1. Cancer Biol Ther 21:452-462 (2020). PubMed: 32089062
- Rachakhom W & Banjerdpongchai R Effect of Calomelanone, a Dihydrochalcone Analogue, on Human Cancer Apoptosis/Regulated Cell Death in an In Vitro Model. Biomed Res Int 2020:4926821 (2020). PubMed: 33415148
- Payton M et al. Dual Targeting of Aurora Kinases with AMG 900 Exhibits Potent Preclinical Activity Against Acute Myeloid Leukemia with Distinct Post-Mitotic Outcomes. Mol Cancer Ther 17:2575-2585 (2018). PubMed: 30266802
- Grammas P et al. Brain microvasculature and hypoxia-related proteins in Alzheimer's disease. Int J Clin Exp Pathol 4:616-27 (2011). WB ; Rat . PubMed: 21904637