Recombinant Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16642] to Bcl-XL - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free
See all Bcl-XL primary antibodies -
Description
Rabbit monoclonal [EPR16642] to Bcl-XL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos, Jurkat, HeLa or K562 whole cell lysate. Mouse brain, heart, kidney, spleen. Rat brain, heart, kidney, spleen. C6, RAW 264.7, PC-12 or NIH/3T3 whole cell lysate. IHC-P: Human kidney, mouse colon, rat testis.
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General notes
ab223547 is the carrier-free version of ab178844.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16642 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis. -
Tissue specificity
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. -
Post-translational
modificationsProteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. -
Cellular localization
Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope. - Information by UniProt
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Database links
- Entrez Gene: 598 Human
- Entrez Gene: 12048 Mouse
- Entrez Gene: 24888 Rat
- Omim: 600039 Human
- SwissProt: Q07817 Human
- SwissProt: Q64373 Mouse
- SwissProt: P53563 Rat
- Unigene: 516966 Human
see all -
Alternative names
- Apoptosis regulator Bcl X antibody
- Apoptosis regulator Bcl-X antibody
- Apoptosis regulator BclX antibody
see all
Images
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All lanes : Anti-Bcl-XL antibody [EPR16642] (ab178844) at 1/20000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDaThis data was developed using ab178844, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab178844, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1:2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab178844, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasm staining on epithelial cells of mouse colon tissue is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab178844, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasm staining on epithelial cells of testis is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab178844, the same antibody clone in a different buffer formulation.
Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration:5% NFDM/TBST. -
All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab178844, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.
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All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution
Lane 1 : Mouse brain
Lane 2 : Mouse heart
Lane 3 : Mouse kidney
Lane 4 : Mouse spleen
Lane 7 : Rat kidney
Lane 8 : Rat spleen
Lane 9 : C6 whole cell lysates
Lane 10 : Raw264.7 whole cell lysates
Lane 11 : PC-12 whole cell lysates
Lane 12 : NIH/3T3 whole cell lysates
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab178844, the same antibody clone in a different buffer formulation.
This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab178844, the same antibody clone in a different buffer formulation.
This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Rat testis tissue.
Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasm staining on epithelial cells of testis is observed.
Negative control: Using PBS instead of primary ab, secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab178844, the same antibody clone in a different buffer formulation.
This immunoprecipitation image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.
Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.
Blocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab223547 has not yet been referenced specifically in any publications.