Overview

  • Product name
    Anti-Bcl10 antibody [4F8E8H8]
    See all Bcl10 primary antibodies
  • Description
    Mouse monoclonal [4F8E8H8] to Bcl10
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • MCF-7 cell extract. Human breast and liver carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab36585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/1000.
WB 1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/200.
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similarities
    Contains 1 CARD domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localization
    Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI132454 antibody
    • B cell CLL/lymphoma 10 antibody
    • B cell lymphoma/leukemia10 antibody
    • B-cell CLL/lymphoma 10 antibody
    • B-cell leukemia/lymphoma 10 antibody
    • B-cell lymphoma/leukemia 10 antibody
    • Bcl 10 antibody
    • Bcl-10 antibody
    • Bcl10 antibody
    • BCL10_HUMAN antibody
    • c E10 antibody
    • c-E10 antibody
    • C81403 antibody
    • CARD containing apoptotic signaling protein antibody
    • CARD containing molecule enhancing NF kappa B antibody
    • CARD containing molecule enhancing NF kB antibody
    • CARD containing molecule enhancing NF-kB antibody
    • CARD containing molecule enhancing NFkB antibody
    • CARD containing proapoptotic protein antibody
    • CARD like apoptotic protein antibody
    • CARD-containing apoptotic signaling protein antibody
    • CARD-containing molecule enhancing NF-kappa-B antibody
    • CARD-containing proapoptotic protein antibody
    • CARD-like apoptotic protein antibody
    • CARMEN antibody
    • Caspase recruiting domain containing protein antibody
    • caspase-recruiting domain-containing protein antibody
    • cCARMEN antibody
    • cE 10 antibody
    • cE10 antibody
    • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
    • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
    • CED3/ICH1 prodomain homologous E10 like regulator antibody
    • Cellular E10 antibody
    • Cellular homolog of vCARMEN antibody
    • Cellular-E10 antibody
    • CIPER antibody
    • CLAP antibody
    • hCLAP antibody
    • Mammalian CARD containing adapter molecule E10 antibody
    • Mammalian CARD-containing adapter molecule E10 antibody
    • mE 10 antibody
    • mE10 antibody
    • R-RCD1 antibody
    see all

Images

  • All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution

    Lane 1 : Cell lysates prepared from NIH/3T3 cells
    Lane 2 : Cell lysates prepared from Hela cells
    Lane 3 : Cell lysates prepared from MCF-7 cells
    Lane 4 : Cell lysates prepared from Jurkat cells

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat polyclonal to mouse IgG1

    Predicted band size: 31 kDa

  • Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
  • Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
  • ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.

  • Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).

References

ab36585 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.
The antibody ab36585 was raised agianst the Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3) - thus we do not know where the epitope region is and whether it is N- or C-terminal.
This is the only antibody that has been tested in mouse. Other antibodies were tested in WB, but not mouse or do not react with mouse.

We have ab93604, ab128183, and ab1142 that were raised against a C-terminal region of Bcl10. If you like to try any of these for mouse samples, you can use our Abtrial program.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the to-be-tested product at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount in the amount of the price you paid for the to-be-tested product. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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