Overview

  • Product name

  • Description

    Rabbit polyclonal to Bcl10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Bcl10 (internal sequence). The exact sequence is proprietary.
    Database link: O95999

  • Positive control

    • WB: HeLa cell extract; Raji, Neuro-2a, GL261 and C8D30 whole cell lysates. IP: Raji whole cell extract. IHC-P: Human gastric cancer tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.

Target

  • Function

    Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similarities

    Contains 1 CARD domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localization

    Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI132454 antibody
    • B cell CLL/lymphoma 10 antibody
    • B cell lymphoma/leukemia10 antibody
    • B-cell CLL/lymphoma 10 antibody
    • B-cell leukemia/lymphoma 10 antibody
    • B-cell lymphoma/leukemia 10 antibody
    • Bcl 10 antibody
    • Bcl-10 antibody
    • Bcl10 antibody
    • BCL10_HUMAN antibody
    • c E10 antibody
    • c-E10 antibody
    • C81403 antibody
    • CARD containing apoptotic signaling protein antibody
    • CARD containing molecule enhancing NF kappa B antibody
    • CARD containing molecule enhancing NF kB antibody
    • CARD containing molecule enhancing NF-kB antibody
    • CARD containing molecule enhancing NFkB antibody
    • CARD containing proapoptotic protein antibody
    • CARD like apoptotic protein antibody
    • CARD-containing apoptotic signaling protein antibody
    • CARD-containing molecule enhancing NF-kappa-B antibody
    • CARD-containing proapoptotic protein antibody
    • CARD-like apoptotic protein antibody
    • CARMEN antibody
    • Caspase recruiting domain containing protein antibody
    • caspase-recruiting domain-containing protein antibody
    • cCARMEN antibody
    • cE 10 antibody
    • cE10 antibody
    • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
    • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
    • CED3/ICH1 prodomain homologous E10 like regulator antibody
    • Cellular E10 antibody
    • Cellular homolog of vCARMEN antibody
    • Cellular-E10 antibody
    • CIPER antibody
    • CLAP antibody
    • hCLAP antibody
    • Mammalian CARD containing adapter molecule E10 antibody
    • Mammalian CARD-containing adapter molecule E10 antibody
    • mE 10 antibody
    • mE10 antibody
    • R-RCD1 antibody
    see all

Images

  • All lanes : Anti-Bcl10 antibody (ab227206) at 1/2500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Bcl10 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 26 kDa

  • All lanes : Anti-Bcl10 antibody (ab227206) at 1/2500 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : Bcl10 knockout HeLa cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 26 kDa



    12% SDS-PAGE gel.

  • Anti-Bcl10 antibody (ab227206) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE gel.

  • All lanes : Anti-Bcl10 antibody (ab227206) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12 % SDS-PAGE gel.

  • Bcl10 was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 4 μg ab227206. Western blot was performed from the immunoprecipitate using ab227206 at 1/500 dilution. Anti-rabbit IgG was used as a secondary reagent

    Lane 1: Raji whole cell extract 40 μg (Input).

    Lane 2: Control IP in Raji whole cell extract with 4 μg of preimmune Rabbit IgG.

    Lane 3: ab227206 IP in Raji whole cell extract.

  • Paraffin-embedded human gastric cancer tissue stained for Bcl10 using ab227206 at 1/500 dilution in immunohistochemical analysis.

  • 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Bcl10 (green) using ab227206 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

References

ab227206 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (Spleen)
Permeabilization
Yes - acetone
Gating Strategy
Gated on alive single cells -> CD45+ Bcl10+ -> gate on CD8 and CD19
Specification
Spleen
Preparation
Cell harvesting/tissue preparation method: Harvest spleen, mechanical smash and dissociation, ACK wash, FcR blocking and staining
Sample buffer: MACS buffer
Fixation
Acetone

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Verified customer

Submitted Nov 14 2018

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