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    bcl10-antibody-epr3174-ab108412.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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RecombinantRabMAb

Recombinant Anti-Bcl10 antibody [EPR3174] (ab108412)

  • Datasheet
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Western blot - Anti-Bcl10 antibody [EPR3174] (ab108412)
  • Flow Cytometry (Intracellular) - Anti-Bcl10 antibody [EPR3174] (ab108412)
  • Anti-Bcl10 antibody [EPR3174] (ab108412)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3174] to Bcl10
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Bcl10 antibody [EPR3174]
    See all Bcl10 primary antibodies
  • Description

    Rabbit monoclonal [EPR3174] to Bcl10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Daudi and Raji cell lysates; Raji cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3174
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Bcl10 antibody [EPR3174] - BSA and Azide free (ab247661)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji membrane extract lysate (ab30126)
  • Recombinant Protein

    • Recombinant Human Bcl10 protein (ab124575)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
Flow Cyt (Intra)
1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
Flow Cyt (Intra)
1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for ICC,IHC-P or IP.

Target

  • Function

    Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similarities

    Contains 1 CARD domain.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localization

    Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Target information above from: UniProt accession O95999 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8915 Human
    • Omim: 603517 Human
    • SwissProt: O95999 Human
    • Unigene: 193516 Human
    • Alternative names

      • AI132454 antibody
      • B cell CLL/lymphoma 10 antibody
      • B cell lymphoma/leukemia10 antibody
      • B-cell CLL/lymphoma 10 antibody
      • B-cell leukemia/lymphoma 10 antibody
      • B-cell lymphoma/leukemia 10 antibody
      • Bcl 10 antibody
      • Bcl-10 antibody
      • Bcl10 antibody
      • BCL10_HUMAN antibody
      • c E10 antibody
      • c-E10 antibody
      • C81403 antibody
      • CARD containing apoptotic signaling protein antibody
      • CARD containing molecule enhancing NF kappa B antibody
      • CARD containing molecule enhancing NF kB antibody
      • CARD containing molecule enhancing NF-kB antibody
      • CARD containing molecule enhancing NFkB antibody
      • CARD containing proapoptotic protein antibody
      • CARD like apoptotic protein antibody
      • CARD-containing apoptotic signaling protein antibody
      • CARD-containing molecule enhancing NF-kappa-B antibody
      • CARD-containing proapoptotic protein antibody
      • CARD-like apoptotic protein antibody
      • CARMEN antibody
      • Caspase recruiting domain containing protein antibody
      • caspase-recruiting domain-containing protein antibody
      • cCARMEN antibody
      • cE 10 antibody
      • cE10 antibody
      • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
      • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
      • CED3/ICH1 prodomain homologous E10 like regulator antibody
      • Cellular E10 antibody
      • Cellular homolog of vCARMEN antibody
      • Cellular-E10 antibody
      • CIPER antibody
      • CLAP antibody
      • hCLAP antibody
      • Mammalian CARD containing adapter molecule E10 antibody
      • Mammalian CARD-containing adapter molecule E10 antibody
      • mE 10 antibody
      • mE10 antibody
      • R-RCD1 antibody
      see all

    Images

    • Western blot - Anti-Bcl10 antibody [EPR3174] (ab108412)
      Western blot - Anti-Bcl10 antibody [EPR3174] (ab108412)
      All lanes : Anti-Bcl10 antibody [EPR3174] (ab108412) at 1/1000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Flow Cytometry (Intracellular) - Anti-Bcl10 antibody [EPR3174] (ab108412)
      Flow Cytometry (Intracellular) - Anti-Bcl10 antibody [EPR3174] (ab108412)

      Intracellular flow cytometric analysis of Bcl10 in permeabilized Raji cells, using ab108412 at a 1/500 dilution (red) or a Rabbit IgG (negative) (green).

       

    • Anti-Bcl10 antibody [EPR3174] (ab108412)
      Anti-Bcl10 antibody [EPR3174] (ab108412)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab108412? Please let us know so that we can cite the reference in this datasheet.

    ab108412 has not yet been referenced specifically in any publications.

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