Recombinant Anti-Bcl10 antibody [EPR3174] (ab108412)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Bcl10 antibody [EPR3174]
See all Bcl10 primary antibodies -
Description
Rabbit monoclonal [EPR3174] to Bcl10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Bcl10 aa 50-150. The exact sequence is proprietary.
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Positive control
- Daudi and Raji cell lysates; Raji cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3174 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 26 kDa. | |
| Flow Cyt | 1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similarities
Contains 1 CARD domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
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Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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![Western blot - Anti-Bcl10 antibody [EPR3174] (ab108412)](https://www.abcam.com/ps/products/108/ab108412/Images/Bcl10-Primary-antibodies-ab108412-1.jpg)
Western blot - Anti-Bcl10 antibody [EPR3174] (ab108412)All lanes : Anti-Bcl10 antibody [EPR3174] (ab108412) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Flow Cytometry - Anti-Bcl10 antibody [EPR3174] (ab108412)](https://www.abcam.com/ps/products/108/ab108412/Images/Bcl10-Primary-antibodies-ab108412-2.jpg)
Flow Cytometry - Anti-Bcl10 antibody [EPR3174] (ab108412)Flow cytometric analysis of Bcl10 in permeabilized Raji cells, using ab108412 at a 1/500 dilution (red) or a Rabbit IgG (negative) (green).
Datasheets and documents
References
ab108412 has not yet been referenced specifically in any publications.
