Recombinant
RabMAb

Anti-Bcl10 antibody [EPR3174] (ab108412)

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Overview

  • Product name
    Anti-Bcl10 antibody [EPR3174]
    See all Bcl10 primary antibodies
  • Description
    Rabbit monoclonal [EPR3174] to Bcl10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Bcl10 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Daudi and Raji cell lysates; Raji cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function
      Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
    • Tissue specificity
      Ubiquitous.
    • Involvement in disease
      Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
      Note=Defects in BCL10 are involved in various types of cancer.
    • Sequence similarities
      Contains 1 CARD domain.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
    • Cellular localization
      Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
      • Alternative names
        • AI132454 antibody
        • B cell CLL/lymphoma 10 antibody
        • B cell lymphoma/leukemia10 antibody
        • B-cell CLL/lymphoma 10 antibody
        • B-cell leukemia/lymphoma 10 antibody
        • B-cell lymphoma/leukemia 10 antibody
        • Bcl 10 antibody
        • Bcl-10 antibody
        • Bcl10 antibody
        • BCL10_HUMAN antibody
        • c E10 antibody
        • c-E10 antibody
        • C81403 antibody
        • CARD containing apoptotic signaling protein antibody
        • CARD containing molecule enhancing NF kappa B antibody
        • CARD containing molecule enhancing NF kB antibody
        • CARD containing molecule enhancing NF-kB antibody
        • CARD containing molecule enhancing NFkB antibody
        • CARD containing proapoptotic protein antibody
        • CARD like apoptotic protein antibody
        • CARD-containing apoptotic signaling protein antibody
        • CARD-containing molecule enhancing NF-kappa-B antibody
        • CARD-containing proapoptotic protein antibody
        • CARD-like apoptotic protein antibody
        • CARMEN antibody
        • Caspase recruiting domain containing protein antibody
        • caspase-recruiting domain-containing protein antibody
        • cCARMEN antibody
        • cE 10 antibody
        • cE10 antibody
        • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
        • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
        • CED3/ICH1 prodomain homologous E10 like regulator antibody
        • Cellular E10 antibody
        • Cellular homolog of vCARMEN antibody
        • Cellular-E10 antibody
        • CIPER antibody
        • CLAP antibody
        • hCLAP antibody
        • Mammalian CARD containing adapter molecule E10 antibody
        • Mammalian CARD-containing adapter molecule E10 antibody
        • mE 10 antibody
        • mE10 antibody
        • R-RCD1 antibody
        see all

      Images

      • Western blot - Anti-Bcl10 antibody [EPR3174] (ab108412)
        Western blot - Anti-Bcl10 antibody [EPR3174] (ab108412)
        All lanes : Anti-Bcl10 antibody [EPR3174] (ab108412) at 1/1000 dilution

        Lane 1 : Daudi cell lysate
        Lane 2 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 26 kDa

      • Flow Cytometry - Anti-Bcl10 antibody [EPR3174] (ab108412)
        Flow Cytometry - Anti-Bcl10 antibody [EPR3174] (ab108412)
        Flow cytometric analysis of Bcl10 in permeabilized Raji cells, using ab108412 at a 1/500 dilution (red) or a Rabbit IgG (negative) (green).

      References

      ab108412 has not yet been referenced specifically in any publications.

      Publishing research using ab108412? Please let us know so that we can cite the reference in this datasheet.

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