Recombinant
RabMAb

Recombinant Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free (ab236221)

Overview

  • Product name

    Anti-Bcl2 alpha antibody [SP66] - BSA and Azide free
    See all Bcl2 alpha primary antibodies
  • Description

    Rabbit monoclonal [SP66] to Bcl2 alpha - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Bcl2 alpha aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: P10415

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab236221 is a PBS-only buffer format of ab93884. Please refer to ab93884 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • Relevance

    BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
  • Cellular localization

    Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
  • Database links

  • Alternative names

    • Apoptosis regulator Bcl2 antibody
    • B cell lymphoma protein 2 alpha isoform antibody
    • B cell lymphoma protein 2 antibody
    • B-cell CLL/lymphoma 2 antibody
    • B-cell leukemia/lymphoma 2 antibody
    • Bcl 2 antibody
    • Leukemia /lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    see all

Images

  • Flow cytometric analysis of MCF7 (human T cell leukemia cell line from peripheral blood) cell line labeling Bcl2 alpha with ab93884 at 1/100 (green) compared with a rabbit negative control (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93884).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bcl2 alpha with ab93884 at 1/100 dilution in immunohistochemical analysis. DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab93884).

References

ab236221 has not yet been referenced specifically in any publications.

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